MRE11 complex links RECQ5 helicase to sites of DNA damage

0333644 - ÚMG 2010 RIV GB eng J - Článek v odborném periodiku
Zheng, L. - Kanagaraj, R. - Mihaljevic, B. - Schwendener, S. - Sartori, A.A. - Gerrits, B. - Shevelev, Igor - Janščák, Pavel
MRE11 complex links RECQ5 helicase to sites of DNA damage.
Nucleic Acids Research. Roč. 37, č. 8 (2009), s. 2645-2657. ISSN 0305-1048. E-ISSN 1362-4962
Grant CEP: GA ČR GA204/09/0565
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: homologous recombination, * RECQ5 helicase * MRE11 * DNA repair
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 7.479, rok: 2009

RECQ5 DNA helicase suppresses homologous recombination (HR) possibly through disruption of RAD51 filaments. We show that RECQ5 is constitutively associated with the MRE11-RAD50-NBS1 (MRN) complex, a primary sensor of DNA double-strand breaks (DSBs) that promotes DSB repair and regulates DNA damage signaling via activation of ATM kinase. Experiments indicated that RECQ5 interacts with the MRN complex through both MRE11 and NBS1, and that RECQ5 specifically inhibited the 3´-5´ exonuclease activity of MRE11, while MRN had no effect on the helicase activity of RECQ5. At the cellular level, we observed that the MRN complex was required for recruitment of RECQ5 to sites of DNA damage. Accumulation of RECQ5 at DSBs was neither dependent on MDC1 that mediates binding of MRN to DSB-flanking chromatin nor on CtIP that acts in conjunction with MRN to promote resection of DSBs for repair by HR. These data suggest that the MRN complex recruits RECQ5 to sites of DNA damage to regulate DNA repair.
Trvalý link: http://hdl.handle.net/11104/0178583