Tetrathiafulvalene-labelled nucleosides and nucleoside triphosphates. Synthesis, electrochemistry and scope of their polymerase incorporation into DNA

0328740 - ÚOCHB 2010 RIV DE eng J - Článek v odborném periodiku
Riedl, Jan - Horáková Brázdilová, Petra - Šebest, Peter - Pohl, Radek - Havran, Luděk - Fojta, Miroslav - Hocek, Michal
Tetrathiafulvalene-labelled nucleosides and nucleoside triphosphates. Synthesis, electrochemistry and scope of their polymerase incorporation into DNA.
European Journal of Organic Chemistry. -, č. 21 (2009), s. 3519-3525. ISSN 1434-193X. E-ISSN 1099-0690
Grant CEP: GA AV ČR(CZ) IAA400040901
Výzkumný záměr: CEZ:AV0Z40550506; CEZ:AV0Z50040507
Klíčová slova: nucleoside triphosphates * DNA polymerase * tetrathiafulvalene
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 3.096, rok: 2009

DNA labeling by tetrathiafulvalene was attempted. TTF-modified nucleoside triphosphates were prepared and tested as substrates for DNA polymerases. Their enzymatic incorporation was inefficient and at higher concentration they fully inhibited the polymerase.
Trvalý link: http://hdl.handle.net/11104/0174977