Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods

0326498 - ÚOCHB 2010 RIV US eng J - Článek v odborném periodiku
Klusák, Vojtěch - Bařinka, C. - Plechanovová, Anna - Mlčochová, Petra - Konvalinka, Jan - Rulíšek, Lubomír - Lubkowski, J.
Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods.
Biochemistry. Roč. 48, č. 19 (2009), s. 4126-4138. ISSN 0006-2960
Grant CEP: GA MŠMT 1M0508; GA MŠMT LC512
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: reaction mechanism * QM/MM calculations * glutamate carboxypeptidase II * X-ray crystallography * mutant activity
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 3.226, rok: 2009

The peptide hydrolysis by GCPII was investigated in detail by carrying out the kinetic analysis of GCPII(E424A) using N-Ac-Asp-Glu as substrate. The crystal structure of GCPII(E424A) in complex with N-Ac-Asp-Glu was determined at 1.70 A resolution. The experimental data were then complemented by the QM/MM calculations which provided detailed information concerning the GCPII reaction mechanism.
Trvalý link: http://hdl.handle.net/11104/0173587