Plant antigens cross-react with rat polyclonal antibodies against

0318781 - ÚEB 2009 RIV GB eng J - Článek v odborném periodiku
Oulehlová, Denisa - Hála, Michal - Potocký, Martin - Žárský, Viktor - Cvrčková, F.
Plant antigens cross-react with rat polyclonal antibodies against.
[Rostlinné antigeny křížově reagují s potkaními protilátkami proti peptidům konjugovaným s KLH.]
Cell Biology International. Roč. 33, č. 1 (2009), s. 113-118. ISSN 1065-6995. E-ISSN 1095-8355
Grant CEP: GA AV ČR KJB600380601; GA ČR GA204/05/0268; GA MŠMT(CZ) LC06004; GA MŠMT(CZ) LC06034
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Keyhole limpet hemocyanin * Rat antisera * Synthetic peptides
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.800, rok: 2009

Keyhole limpet hemocyanin (KLH)-conjugated peptides are routinely used to raise polyclonal antibodies for biochemical or immunolocalization studies. Rats are suitable for producing antisera against plant antigens as they often lack non-specific response towards plant materials. We attempted to obtain rat antisera against peptides derived from several plant proteins. However, most antisera recognized the same background KLH-related plant antigen (KRAP) in Arabidopsis and tobacco. We characterized KRAP with respect to size and cellular localization and examined possible antigen-specific reasons for the failure of most immunizations. We also found no reports of successful use of rat anti-KLH-peptide antibodies in plant studies. We thus believe that the rat-KLH:peptide system is poorly suited for production of antibodies, especially against plant antigens, and should be used with caution, if at all.

Použili jsme různé peptidy pro přípravu protilátek v potkanech. Ve všech případech rozeznávaly rostlinně specifický antigen - KRAP. Ukazujeme, že potkan není vhodný pro přípravu protilátek proti peptidům konjugovaným s KLH, pro konjugace běžně používaným proteinem.
Trvalý link: http://hdl.handle.net/11104/0168098