0616560 - MBÚ 2026 GB eng J - Článek v odborném periodiku
Neuhöferová, Eva - Kindermann, Marek - Buzgo, Matej - Vocetková, Karolína - Pánek, D. - Cígler, Petr - Benson, Veronika
Topical siRNA therapy of diabetic-like wound healing.
Journal of Materials Chemistry B. Roč. 13, č. 3 (2024), s. 1037-1051. ISSN 2050-750X. E-ISSN 2050-7518
Grant CEP: GA ČR(CZ) GA23-04876S; GA TA ČR(CZ) TH90010001; GA MŠMT EH22_008/0004558
GRANT EU: European Commission(XE) 101135359 - C-QuENS; European Commission(XE) 101086142 - FLORIN
Grant ostatní: AV ČR(CZ) StrategieAV21/29
Program: StrategieAV
Institucionální podpora: RVO:61388971 ; RVO:61388963 ; RVO:68378041
Klíčová slova: fluorescent nanodiamonds * controlled-release * delivery * degradation * nanofibers * dressings * membranes
Obor OECD: Microbiology; Biomaterials (as related to medical implants, devices, sensors) (UEM-P); Nano-materials (production and properties) (UOCHB-X)
Impakt faktor: 6.1, rok: 2023 ; AIS: 0.952, rok: 2023
Způsob publikování: Open access
Web výsledku:
https://pubs.rsc.org/en/content/articlelanding/2025/tb/d4tb01547aDOI: https://doi.org/10.1039/d4tb01547a

Non-healing wounds are a serious complication in diabetic patients. One of the detrimental factors contributing to limited wound healing is the accumulation of metalloproteinase-9 (MMP-9) in the wound. Selective inhibition of MMP-9 is one of the established therapeutic targets for diabetic wound healing. Here, a functional and biocompatible wound dressing is developed to enable a controlled release of a traceable vector loaded with the antisense siRNA against MMP-9 in the wound. The dressing consists of degradable polymer nanofibers embedded with a vector nanosystem polymer-coated fluorescent nanodiamonds optimized for the binding of siRNA and colloidal stability of nanodiamond-siRNA complexes in a physiological environment. The developed dressing is tested on murine fibroblasts and also applied to wounds in a diabetic murine model to evaluate its suitability in terms of in vivo toxicity, biological efficacy, and handling. The treatment results in significant local inhibition of MMP-9 and a shortening of the wound healing time. The scar formation in treated diabetic-like mice becomes comparable with that in non-treated diabetes-free mice. Our results suggest that the application of our biocompatible dressing loaded with a non-toxic vector nanosystem is an effective and promising approach to gene therapy of non-healing wounds.
Trvalý link: https://hdl.handle.net/11104/0363552