Počet záznamů: 1
Encapsulating melittin from animal venom by finely tuned charge compensation with polymer carriers
- 1.
SYSNO ASEP 0570496 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Encapsulating melittin from animal venom by finely tuned charge compensation with polymer carriers Tvůrce(i) Černochová, Zulfiya (UMCH-V) RID, ORCID
Lobaz, Volodymyr (UMCH-V) RID, ORCID
Čtveráčková, Lucie (UMCH-V)
Černoch, Peter (UMCH-V) RID, ORCID
Šlouf, Miroslav (UMCH-V) RID, ORCID
Filipová, Marcela (UMCH-V) RID, ORCID
Hrubý, Martin (UMCH-V) RID, ORCID
Pánek, Jiří (UMCH-V) RID, ORCIDČíslo článku 111996 Zdroj.dok. European Polymer Journal. - : Elsevier - ISSN 0014-3057
Roč. 190, 25 May (2023)Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova melittin ; encapsulation ; polyanion Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science CEP GA21-04166S GA ČR - Grantová agentura ČR GC20-15479J GA ČR - Grantová agentura ČR LM2023053 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000958643200001 EID SCOPUS 85150444923 DOI https://doi.org/10.1016/j.eurpolymj.2023.111996 Anotace Due to their wide availability, polypeptide/protein venoms produced by certain insects are of high interest as potential active pharmacological ingredients. However, the number of clinical studies on these venoms remains poor. In this paper, we describe an extremely efficient platform to form polyplexes from cationic amphiphilic animal venoms, such as the hemolytic poison melittin. The idea is based on supramolecular melittin cationic charge compensation with nanoparticles (micelles composed of hydrophobic glassy polystyrene core and hydrophilic poly(meth)acrylic acid corona, PMA and PAA acid homopolymers) with high anion charge density at physiological pH. An instant “mix and go” process and efficient melittin complexation with nanoparticles at concentrations down to micrograms per milliliter of PBS solution was achieved and proven by inhibition of melittin-induced hemolysis. We studied in detail how the structural features of the nanoparticles influence their potency, we found that the most important parameter is the number of carboxylates in the polyanion chain (proportional to the molecular weight and length of the anionic poly(meth)acrylic acid block). The poly(meth)acrylic acid-based polymer nanoparticles may be useful as melittin (and possibly other cationic amphiphilic animal venoms) antidotes but also as models for constructing future delivery systems to apply the venoms therapeutically. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2024 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0014305723001799?via%3Dihub
Počet záznamů: 1