Počet záznamů: 1  

Assembly for Cryo-SEM/Raman Microspectroscopy sample analysis

  1. 1.
    SYSNO ASEP0568569
    Druh ASEPA - Abstrakt
    Zařazení RIVO - Ostatní
    NázevAssembly for Cryo-SEM/Raman Microspectroscopy sample analysis
    Tvůrce(i) Láznička, Tomáš (UPT-D) ORCID, RID, SAI
    Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
    Ježek, Jan (UPT-D) RID, ORCID, SAI
    Kizovský, Martin (UPT-D)
    Bernatová, Silvie (UPT-D) RID, SAI
    Mrázová, Kateřina (UPT-D) SAI, ORCID, RID
    Obruča, S. (CZ)
    Zemánek, Pavel (UPT-D) RID, SAI, ORCID
    Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
    Zdroj.dok.16th Multinational Congress on Microscopy, 16MCM, 04-09 September 2022, Brno, Czech Republic. Book of abstracts. - Brno : Czechoslovak Microscopy Society, 2022 / Krzyžánek V. ; Hrubanová K. ; Hozák P. ; Müllerová I. ; Šlouf M. - ISBN 978-80-11-02253-2
    S. 63-64
    Poč.str.2 s.
    Forma vydáníOnline - E
    AkceMultinational Congress on Microscopy /16./
    Datum konání04.09.2022 - 09.09.2022
    Místo konáníBrno
    ZeměCZ - Česká republika
    Typ akceWRD
    Jazyk dok.eng - angličtina
    Země vyd.CZ - Česká republika
    Klíč. slovaCryo-SEM ; Raman spectroscopy ; electron microscopy
    Vědní obor RIVJA - Elektronika a optoelektronika, elektrotechnika
    Obor OECDElectrical and electronic engineering
    CEPTN01000008 GA TA ČR - Technologická agentura ČR
    Institucionální podporaUPT-D - RVO:68081731
    AnotaceCorrelative imaging using different methods of observation is now becoming more and more popular. It allows the interconnection of various information of the sample accessible only by
    individual techniques. It is crucial to find and observe the same place on the sample. The same objects and their locations (cell shape and arrangement) can be correlated with each other,
    especially with software when 2 different imaging devices were used. Combination of the Cryo-Scanning Electron Microscopy (Cryo-SEM) and Cryo-Raman Micro-spectroscopy (Cryo-Raman) gives many benefits. Surface, structure or morphology of the sample can by studied using Cryo-SEM, and detection and identification of elements or compounds in the examined sample using Cryo-Raman. For observation of various, especially biological samples, their freezing to the temperature of liquid nitrogen is used. The motivation was to design an assembly for motion and observation using Cryo-Raman and its connection with commercial holders used for the observation of vitrified samples by Cryo-SEM. Assembly allows movement of the sample to a known position and measuring the cryogen level using LabVIEW software. Our assembly consists of a Dewar vessel, which contains liquid nitrogen. The stage is inside the Dewar vessel below the liquid level and is made by 3D printing and enables 3 positions for individual types of sample holders (Leica microsystems, Gatan and Quorum technologies). The Dewar vessel lies on piezoelectric stage allowing movement in both X and Y direction. The stage is connected to the Raman spectrometer table providing displacement in the Z direction. The Dewar vessel is covered with Plexiglas to minimize contamination. Assembly was tested on dried samples and polystyrene in cryo temperatures with known spectrum to verify functionality. Then biological samples including C. necator H16 were tested. System allows more efficient correlation between individual analyses of microorganisms containing biopolymer particles. For example, several representatives of soil microorganisms, such as bacteria C. necator H16 which are able to produce polyhydroxyalkanoates (PHA). PHAs are polyesters of hydroxyalkanoic acids, which serve as energy storage. PHAs attract attention as an alternative to petrochemical plastics, which can be biotechnologically produced from waste streams of some industrial processes. It was proved that the combination of Raman spectroscopy with the cryo-SEM technique can provide a deeper insight into the chemical and mechanical properties of polymeric granules inside the bacterial cells and it will serve for future research.
    PracovištěÚstav přístrojové techniky
    KontaktMartina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
    Rok sběru2023
    Elektronická adresahttps://www.16mcm.cz/wp-content/uploads/2022/09/16MCM-abstract-book.pdf
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.