Počet záznamů: 1
Acinetobacter amyesii sp. nov., widespread in the soil and water environment and animals
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SYSNO ASEP 0564932 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Acinetobacter amyesii sp. nov., widespread in the soil and water environment and animals Tvůrce(i) Němec, A. (CZ)
Radolfová-Křížová, L. (CZ)
Maixnerová, M. (CZ)
Němec, M. (CZ)
Shestivska, V. (CZ)
Španělová, P. (CZ)
Kyselková, Martina (MBU-M) ORCID
Wilharm, G. (DE)
Higgins, P. G. (DE)Číslo článku 005642 Zdroj.dok. International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society - ISSN 1466-5026
Roč. 72, č. 10 (2022)Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova carbon source assimilation ; core genome phylogeny ; maldi-tof ms ; whole genome sequence Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA22-05373S GA ČR - Grantová agentura ČR Výzkumná infrastruktura e-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000800144400006 EID SCOPUS 85140855283 DOI https://doi.org/10.1099/ijsem.0.005642 Anotace We studied a novel taxon of the genus Acinetobacter , which comprised six strains collected in Czechia, Germany, Indonesia and Turkey between 2015 and 2021. The organisms were isolated from environmental soil, water samples and cow faeces. Their genome sizes varied between 3.3 and 3.5 Mb, with a G+C content of 40.4–40.8 mol%. Based on genus-wide core genome analysis, the taxon formed a distinct clade, with Acinetobacter gandensis being the phylogenetically closest related species. The intrataxon genomic average nucleotide identity based on blast (ANIb) and digital DNA–DNA hybridization (dDDH) values reached 95.3–97.4% and 62.5–77.8 %, respectively, whereas its ANIb/dDDH values against the known Acinetobacter type strains were ≤82.7 %/≤25.7 %. Cluster analysis of whole-cell MALDI-TOF mass spectra corroborated the distinctness and cohesiveness of the taxon. The novel strains were non-glucose-oxidizing, non-haemolytic and non-proteolytic, growing at up to 37–41 °C but not at 44 °C and utilizing 8–10 of the 36 carbon sources tested. Growth on glutarate, tricarballylate and at 37 °C combined with the inability to assimilate 4-aminobutyrate and d-malate differentiated them from all validly named Acinetobacter species. The inspection of genome sequences in the NCBI database revealed the existence of numerous strains conspecific with this group, which were collected from pig faeces and environmental samples in China. We conclude that the taxon represents an ecologically and geographically widespread species, for which we propose the name Acinetobacter amyesii sp. nov., with ANC 5579T (= CCM 9242T=CCUG 76274T=CNCTC 8134T) as the type strain. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005642
Počet záznamů: 1