Počet záznamů: 1
Torpor/hibernation cycle may enhance the risk of insecticides for bats: an in vitro study
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SYSNO ASEP 0555569 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Torpor/hibernation cycle may enhance the risk of insecticides for bats: an in vitro study Tvůrce(i) Kaňová, M. (CZ)
Banďouchová, H. (CZ)
Abdelsalam, E. E. E. (CZ)
Linhart, P. (CZ)
Sedláčková, J. (CZ)
Seidlová, V. (CZ)
Zukal, Jan (UBO-W) RID, ORCID, SAI
Pikula, J. (CZ)Celkový počet autorů 8 Zdroj.dok. Acta veterinaria Brno. - : Veterinární a farmaceutická univerzita Brno - ISSN 0001-7213
Roč. 91, č. 1 (2022), s. 59-68Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova Bats ; Nyctalus noctula ; hibernation ; permethrin ; imidacloprid ; cytotoxicity Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína Obor OECD Veterinary science Způsob publikování Open access Institucionální podpora UBO-W - RVO:68081766 UT WOS 000752524500007 EID SCOPUS 85124456600 DOI 10.2754/avb202291010059 Anotace Exposure to pollutants is considered one of the potential reasons of population declines in bats. In the context of previous studies, we managed to create and keep a wide collection of cell lines from European bat species. Liver cells were chosen for testing, as they represent the preferred model for toxicological studies. Bats are protected, cell lines replacing experimental animals thus represent a unique opportunity to examine effects of pollutants which animals are exposed to in their environments. Moreover, cell incubation temperature variation may simulate physiological states of heterothermic bats. Liver cell lines were cultivated to the required cell number. Exposure to five different concentrations of permethrin (PM) and imidacloprid (IMI) were used to determine cytotoxic effects of these pesticides on Nyctalus noctula-derived liver cells cultivated at 37 degrees C and 8 degrees C for 24 h. An assay based on the measurement of activity of lactate dehydrogenase released from damaged cells was used for quantitating cytotoxicity. Cytotoxicity of IMI ranged from 0% to 47% and from 56% to 67% at 37 degrees C and 8 degrees C, respectively. Cytotoxicity of PM ranged from 36% to 56% and from 43% to 88% at 37 degrees C and 8 degrees C, respectively. Permethrin was tested on the cells at an order of magnitude lower concentrations, even so, higher degree of cytotoxicity was recorded. Imidacloprid was more toxic to bat liver cells at a hibernation temperature than at body temperature of 37 degrees C. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2023 Elektronická adresa https://actavet.vfu.cz/91/1/0059/
Počet záznamů: 1