Počet záznamů: 1
SEC6 exocyst subunit contributes to multiple steps of growth and development of Physcomitrella (Physcomitrium patens)
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SYSNO ASEP 0547992 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název SEC6 exocyst subunit contributes to multiple steps of growth and development of Physcomitrella (Physcomitrium patens) Tvůrce(i) Brejšková, Lucie (UEB-Q) ORCID
Hála, Michal (UEB-Q) RID, ORCID
Rawat, Anamika (UEB-Q)
Soukupová, Hana (UEB-Q) RID, ORCID
Cvrčková, F. (CZ)
Charlot, F. (FR)
Nogué, F. (FR)
Haluška, Samuel (UEB-Q) ORCID
Žárský, Viktor (UEB-Q) RID, ORCIDCelkový počet autorů 9 Zdroj.dok. Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 106, č. 3 (2021), s. 831-843Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova cytokinesis ; exocyst ; exocytosis ; gametophore development ; Physcomitrium patens ; polarity ; sec6 Obor OECD Cell biology CEP GA20-11642S GA ČR - Grantová agentura ČR EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000635800800001 EID SCOPUS 85103374184 DOI https://doi.org/10.1111/tpj.15205 Anotace Spatially directed cell division and expansion is important for plant growth and morphogenesis and relies on cooperation between the cytoskeleton and the secretory pathway. The phylogenetically conserved octameric complex exocyst mediates exocytotic vesicle tethering at the plasma membrane. Unlike other exocyst subunits of land plants, the core exocyst subunit SEC6 exists as a single paralog in Physcomitrium patens and Arabidopsis thaliana genomes. Arabidopsis SEC6 (AtSEC6) loss-of-function (LOF) mutation causes male gametophytic lethality. Our attempts to inactivate the P. patens SEC6 gene, PpSEC6, using targeted gene replacement produced two independent partial LOF (‘weak allele’) mutants via perturbation of the PpSEC6 gene locus. These mutants exhibited the same pleiotropic developmental defects: protonema with dominant chloronema stage, diminished caulonemal filament elongation rate, and failure in post-initiation gametophore development. Mutant gametophore buds, mostly initiated from chloronema cells, exhibited disordered cell file organization and cross-wall perforations, resulting in arrested development at the eight- to 10-cell stage. Complementation of both sec6 moss mutant lines by both PpSEC6 and AtSEC6 cDNA rescued gametophore development, including sexual organ differentiation. However, regular sporophyte formation and viable spore production were recovered only by the expression of PpSEC6, whereas the AtSEC6 complementants were only rarely fertile, indicating moss-specific SEC6 functions. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.1111/tpj.15205
Počet záznamů: 1