Počet záznamů: 1
The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020
- 1.
SYSNO ASEP 0533336 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020 Tvůrce(i) Huggett, J.F. (GB)
Whale, A. S. (GB)
De Spiegelaere, W. (BE)
Nour, A. A. (LB)
Bae, Y.-K. (KR)
Benes, V. (DE)
Burke, D. (US)
Cleveland, M. (US)
Corbisier, P. (BE)
Devonshire, A. S. (GB)
Dong, L. (CN)
Drandi, D. (IT)
Foy, C. A. (GB)
Garson, J. A. (US)
He, H.-J. (US)
Hellemans, J. (BE)
Kubista, Mikael (BTO-N) RID, ORCID
Lievens, A. (BE)
Makrigiorgos, M. G. (US)
Milavec, M. (SI)
Mueller, R. D. (US)
Nolan, T. (GB)
O'Sullivan, D. M. (GB)
Pfaffl, M. W. (DE)
Roediger, S. (DE)
Romsos, E. L. (US)
Shipley, G. L. (US)
Taly, V. (FR)
Untergasser, A. (DE)
Wittwer, C. T. (US)
Bustin, S. A. (GB)
Vandesompele, J. (BE)Celkový počet autorů 33 Zdroj.dok. Clinical Chemistry. - : Oxford University Press - ISSN 0009-9147
Roč. 66, č. 8 (2020), s. 1012-1029Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova POLYMERASE-CHAIN-REACTION ; DNA COPY NUMBER ; ABSOLUTE QUANTIFICATION Vědní obor RIV CE - Biochemie Obor OECD Medical laboratory technology (including laboratory samples analysis CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000555400700006 DOI https://doi.org/10.1093/clinchem/hvaa125 Anotace Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges, data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2021 Elektronická adresa https://academic.oup.com/clinchem/article/66/8/1012/5880117
Počet záznamů: 1