Počet záznamů: 1
The role of the microbiome and psychosocial stress in the expression and activity of drug metabolizing enzymes in mice
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SYSNO ASEP 0531243 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The role of the microbiome and psychosocial stress in the expression and activity of drug metabolizing enzymes in mice Tvůrce(i) Zemanová, N. (CZ)
Anzenbacher, P. (CZ)
Zapletalová, I. (CZ)
Jourová, L. (CZ)
Hermanová, Petra (MBU-M) ORCID
Hudcovic, Tomáš (MBU-M) RID, ORCID
Kozáková, Hana (MBU-M) RID, ORCID
Vodička, Martin (FGU-C) RID, ORCID, SAI
Pácha, Jiří (FGU-C) RID, ORCID
Anzenbacherová, E. (CZ)Číslo článku 8529 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 10, č. 1 (2020)Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova inflammation ; health ; PXR Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Fyziologický ústav - Fyziologie CEP GA17-09869S GA ČR - Grantová agentura ČR GA19-08294S GA ČR - Grantová agentura ČR GA18-02993S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; FGU-C - RVO:67985823 UT WOS 000540510300032 EID SCOPUS 85085183583 DOI https://doi.org/10.1038/s41598-020-65595-9 Anotace The gut microbiota is involved in a number of different metabolic processes of the host organism, including the metabolism of xenobiotics. In our study, we focused on liver cytochromes P450 (CYPs), which can metabolize a wide range of exo- and endogenous molecules. We studied changes in mRNA expression and CYP enzyme activities, as well as the mRNA expression of transcription factors that have an important role in CYP expression, all in stressed germ-free (GF) and stressed specific-pathogen-free (SPF) mice. Besides the presence of the gut microbiota, we looked at the difference between acute and chronic stress. Our results show that stress has an impact on CYP mRNA expression, but it is mainly chronic stress that has a significant effect on enzyme activities along with the gut microbiome. In acutely stressed mice, we observed significant changes at the mRNA level, however, the corresponding enzyme activities were not influenced. Our study suggests an important role of the gut microbiota along with chronic psychosocial stress in the expression and activity of CYPs, which can potentially lead to less effective drug metabolism and, as a result, a harmful impact on the organism. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://www.nature.com/articles/s41598-020-65595-9
Počet záznamů: 1