Počet záznamů: 1
Retroviral host range extension is coupled with Env-activating mutations resulting in receptor-independent entry
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SYSNO ASEP 0486607 Druh ASEP J - Článek v odborném periodiku Zařazení RIV Záznam nebyl označen do RIV Poddruh J Článek ve WOS Název Retroviral host range extension is coupled with Env-activating mutations resulting in receptor-independent entry Tvůrce(i) Lounková, Anna (UMG-J)
Kosla, Jan (UMG-J) RID
Přikryl, David (UMG-J)
Štafl, Kryštof (UMG-J)
Kučerová, Dana (UMG-J)
Svoboda, Jan (UMG-J) RIDCelkový počet autorů 6 Zdroj.dok. Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 114, č. 26 (2017), E5148-E5157Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Rous sarcoma virus ; retrovirus ; virus entry ; envelope glycoprotein ; receptor-independent entry Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Virology CEP GA15-22207S GA ČR - Grantová agentura ČR LO1419 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMG-J - RVO:68378050 UT WOS 000404108400018 DOI 10.1073/pnas.1704750114 Anotace The extent of virus transmission among individuals and species is generally determined by the presence of specific membrane-embedded virus receptors required for virus entry. Interaction of the viral envelope glycoprotein (Env) with a specific cellular receptor is the first and crucial step in determining host specificity. Using a well-established retroviral model-avian Rous sarcoma virus (RSV)-we analyzed changes in an RSV variant that had repeatedly been able to infect rodents. By envelope gene (env) sequencing, we identified eight mutations that do not match the already described mutations influencing the host range. Two of these mutations-one at the beginning (D32G) of the surface Env subunit (SU) and the other at the end of the fusion peptide region (L378S)-were found to be of critical importance, ensuring transmission to rodent, human, and chicken cells lacking the appropriate receptor. Furthermore, we carried out assays to examine the virus entry mechanism and concluded that these two mutations cause conformational changes in the Env variant and that these changes lead to an activated, or primed, state of Env (normally induced after Env interaction with the receptor). In summary, our results indicate that retroviral host range extension is caused by spontaneous Env activation, which circumvents the need for original cell receptor. This activation is, in turn, caused by mutations in various env regions. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2019
Počet záznamů: 1