Počet záznamů: 1
Development of a high throughput single-particle screening for inorganic semiconductor nanorods as neural voltage sensor
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SYSNO ASEP 0485882 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Development of a high throughput single-particle screening for inorganic semiconductor nanorods as neural voltage sensor Tvůrce(i) Kuo, Y. (US)
Park, K. (US)
Li, J. (US)
Ingargiola, A. (US)
Park, J. (US)
Shvadchak, Volodymyr (UOCHB-X) RID, ORCID
Weiss, S. (US)Číslo článku 103520L Zdroj.dok. Proceedings of SPIE. Biosensing and Nanomedicine X, 10352. - Bellingham : SPIE, 2017 / Mohseni H. ; Agahi M. H. ; Razeghi M. - ISSN 0277-786X - ISBN 978-1-5106-1161-0 Poč.str. 8 s. Forma vydání Tištěná - P Akce SPIE Nanoscience + Engineering. Biosensing and Nanomedicine /10./ Datum konání 06.08.2017 - 07.08.2017 Místo konání San Diego Země US - Spojené státy americké Typ akce WRD Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova nanoparticles ; membrane potential ; sensors Vědní obor RIV BO - Biofyzika Obor OECD Biophysics Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000424079800010 EID SCOPUS 85033468963 DOI 10.1117/12.2273089 Anotace Monitoring membrane potential in neurons requires sensors with minimal invasiveness, high spatial and temporal (sub-ms) resolution, and large sensitivity for enabling detection of sub-threshold activities. While organic dyes and fluorescent proteins have been developed to possess voltage-sensing properties, photobleaching, cytotoxicity, low sensitivity, and low spatial resolution have obstructed further studies. Semiconductor nanoparticles (NPs), as prospective voltage sensors, have shown excellent sensitivity based on Quantum confined Stark effect (QCSE) at room temperature and at single particle level. Both theory and experiment have shown their voltage sensitivity can be increased significantly via material, bandgap, and structural engineering. Based on theoretical calculations, we synthesized one of the optimal candidates for voltage sensors: 12 nm type-II ZnSe/CdS nanorods (NRs), with an asymmetrically located seed. The voltage sensitivity and spectral shift were characterized in vitro using spectrally-resolved microscopy using electrodes grown by thin film deposition, which ”sandwich” the NRs. We characterized multiple batches of such NRs and iteratively modified the synthesis to achieve higher voltage sensitivity (Delta F/F>10%), larger spectral shift (>5 nm), better homogeneity, and better colloidal stability. Using a high throughput screening method, we were able to compare the voltage sensitivity of our NRs with commercial spherical quantum dots (QDs) with single particle statistics. Our method of high throughput screening with spectrally-resolved microscope also provides a versatile tool for studying single particles spectroscopy under field modulation. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2018
Počet záznamů: 1