Počet záznamů: 1
Nanoparticle core stability and surface functionalization drive the mTOR signaling pathway in hepatocellular cell lines.
- 1.
SYSNO ASEP 0482038 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nanoparticle core stability and surface functionalization drive the mTOR signaling pathway in hepatocellular cell lines. Tvůrce(i) Lunova, M. (CZ)
Prokhorov, A. (CZ)
Jirsa, M. (CZ)
Hof, M. (CZ)
Olžyńska, A. (CZ)
Jurkiewicz, P. (CZ)
Kubinová, Šárka (UEM-P) RID, ORCID
Lunov, O. (CZ)
Dejneka, A. (CZ)Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 7, č. 1 (2017), s. 16049Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova mesoporous silica nanoparticles ; iron-oxide nanoparticles ; double-stranded-rna ; drug-delivery Vědní obor RIV FP - Ostatní lékařské obory Obor OECD Biophysics CEP LO1309 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 UT WOS 000416118900015 EID SCOPUS 85034824356 DOI https://doi.org/10.1038/s41598-017-16447-6 Anotace Specifically designed and functionalized nanoparticles hold great promise for biomedical applications. Yet, the applicability of nanoparticles is critically predetermined by their surface functionalization and biodegradability. Here we demonstrate that amino-functionalized polystyrene nanoparticles (PS-NH2), but not amino-or hydroxyl-functionalized silica particles, trigger cell death in hepatocellular carcinoma Huh7 cells. Importantly, biodegradability of nanoparticles plays a crucial role in regulation of essential cellular processes. Thus, biodegradable silica nanoparticles having the same shape, size and surface functionalization showed opposite cellular effects in comparison with similar polystyrene nanoparticles. At the molecular level, PS-NH2 obstruct and amino-functionalized silica nanoparticles (Si-NH2) activate the mTOR signalling in Huh7 and HepG2 cells. PS-NH2 induced time-dependent lysosomal destabilization associated with damage of the mitochondrial membrane. Solely in PS-NH2-treated cells, permeabilization of lysosomes preceded cell death. Contrary, Si-NH2 nanoparticles enhanced proliferation of HuH7 and HepG2 cells. Our findings demonstrate complex cellular responses to functionalized nanoparticles and suggest that nanoparticles can be used to control activation of mTOR signaling with subsequent influence on proliferation and viability of HuH7 cells. The data provide fundamental knowledge which could help in developing safe and efficient nano-therapeutics. Pracoviště Ústav experimentální medicíny Kontakt Arzuv Čaryjeva, arzuv.caryjeva@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2018
Počet záznamů: 1