Počet záznamů: 1
Myocardial iron content and mitochondrial function in human heart failure: a direct tissue analysis
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SYSNO ASEP 0477808 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Myocardial iron content and mitochondrial function in human heart failure: a direct tissue analysis Tvůrce(i) Melenovský, V. (CZ)
Petrák, J. (CZ)
Mráček, Tomáš (FGU-C) RID, ORCID
Beneš, J. (CZ)
Borlaug, B. A. (US)
Nůsková, Hana (FGU-C) RID, ORCID
Pluháček, T. (CZ)
Špatenka, J. (CZ)
Kovalčíková, Jana (FGU-C)
Drahota, Zdeněk (FGU-C) RID, ORCID
Kautzner, J. (CZ)
Pirk, J. (CZ)
Houštěk, Josef (FGU-C) RID, ORCIDZdroj.dok. European Journal of Heart Failure - ISSN 1388-9842
Roč. 19, č. 4 (2017), s. 522-530Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova heart failure ; mitochondria ; iron deficiency ; bioenergetics ; metabolism ; reactive oxygen species Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP GB14-36804G GA ČR - Grantová agentura ČR NT14050 GA MZd - Ministerstvo zdravotnictví LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NT14250 GA MZd - Ministerstvo zdravotnictví ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NV16-27496A GA MZd - Ministerstvo zdravotnictví Institucionální podpora FGU-C - RVO:67985823 UT WOS 000401004500011 EID SCOPUS 84988378043 DOI https://doi.org/10.1002/ejhf.640 Anotace Iron replacement improves clinical status in iron-deficient patients with heart failure (HF), but the pathophysiology is poorly understood. The impact of myocardial iron deficiency (MID) on mitochondrial function, measured directly in the failing human heart, is unknown. Left ventricular samples were obtained from 91 consecutive HF patients and 38 HF-free organ donors (controls). Total myocardial iron content, mitochondrial respiration, citric acid cycle and respiratory chain enzyme activities, and content of OXPHOS complexes and ROS-protective enzymes were measured in tissue homogenates to quantify mitochondrial function. Myocardial iron content was lower in HF compared with controls (156 +/- 41 vs. 200 +/- 38 mu g.g(-1) dry weight, P < 0.001), independently of anaemia. MID was associated with more extensive coronary disease and less beta-blocker usage compared with non-MID HF patients. Compared with controls, HF patients displayed reduced myocardial oxygen respiration and reduced activity of all examined mitochondrial enzymes (all P < 0.001). MID in HF was associated with preserved activity of respiratory chain enzymes but reduced activity of aconitase and citrate synthase (by -26% and -15%, P < 0.05) and reduced expression of catalase, glutathione peroxidase, and superoxide dismutase 2. Myocardial iron content is decreased and mitochondrial functions are impaired in advanced HF. MID in HF is associated with diminished citric acid cycle enzyme activities and decreased ROS-protecting enzymes. MID may contribute to altered myocardial substrate use and to worsening of mitochondrial dysfunction that exists in HF. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2018
Počet záznamů: 1