Počet záznamů: 1
Regulating the regulators: responses of four plant growth regulators during clonal propagation of Lachenalia montana
- 1.
SYSNO ASEP 0476488 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Regulating the regulators: responses of four plant growth regulators during clonal propagation of Lachenalia montana Tvůrce(i) Aremu, A.O. (ZA)
Plačková, Lenka (UEB-Q) ORCID, RID
Masondo, N.A. (ZA)
Amoo, S.O. (ZA)
Moyo, M. (ZA)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Doležal, Karel (UEB-Q) RID, ORCID
Van Staden, J. (ZA)Celkový počet autorů 8 Zdroj.dok. Plant Growth Regulation. - : Springer - ISSN 0167-6903
Roč. 82, č. 2 (2017), s. 305-315Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova adventitious bud formation ; zeatin-type cytokinins ; in-vitro ; cv ronina ; endogenous cytokinin ; biological-activity ; bulb production ; south-africa ; biotechnology ; organogenesis ; Asparagaceae ; Floriculture ; Phytohormones ; Physiological disorders ; meta-Topolin ; Ornamentals Vědní obor RIV EF - Botanika Obor OECD Plant sciences, botany CEP LK21306 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA14-34792S GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000399873000009 DOI 10.1007/s10725-017-0260-9 Anotace Lachenalia species are endemic southern African plants with narrow geographical distribution, and are well-traded as ornamental plants in the international floriculture industry. In an attempt to have a better understanding of their growth and hormonal physiology, we evaluated the effects of different plant growth regulators (PGRs) during the clonal regeneration of Lachenalia montana. An auxin (alpha-naphthaleneacetic acid = NAA) and three cytokinin (CK) types (benzyladenine = BA, meta-topolin riboside = mTR and isopentenyladenine = iP), each at three concentrations (1, 5 and 10 A mu M), were tested and the effect of these PGRs on the accumulation of endogenous CK metabolites was evaluated to provide clues on the observed morphological responses. As the most efficient PGR, 10 A mu M mTR treatment produced the highest number of shoots (approximately five shoots per explant) while 1 A mu M BA-treated plants had more bulbs (approximately three bulbs per plantlet). Rooting was generally lower with increasing concentration of PGRs especially with the aromatic-type CKs. Based on the concentrations of endogenous CKs, 10 A mu M mTR regenerants also had the highest CKs (40 142.5 pmol g(-1) DW) which were mainly of the aromatic-type (98%). In terms of the functional role of the CKs, O-glucosides (which are reversible CK storage forms) were the most dominant CK-type in the regenerants from 10 A mu M mTR treatment. On the other hand, the poor rooting, mostly prominent in regenerants from BA treatments was closely related to the high accumulation of N (9)-glucosides (well-known CK metabolites directly involved in rooting inhibition) when compared to regenerants from other treatments. Overall, the current findings provide evidence on the interrelationship existing among the exogenous PGRs, phenotypic responses and the endogenous CKs in the in vitro regenerants. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2018
Počet záznamů: 1