0471446 - ÚEM 2017 RIV GB eng J - Journal Article
Lunova, M. - Zablotskyy, V. - Dempsey, N.M. - Devillers, T. - Jirsa, M. - Syková, Eva - Kubinová, Šárka - Lunov, O. - Dejneka, A.
Modulation of collective cell behaviour by geometrical constraints.
Integrative Biology. Roč. 8, č. 11 (2016), s. 1099-1110. ISSN 1757-9694. E-ISSN 1757-9708
R&D Projects: GA MŠMT(CZ) LO1309
Institutional support: RVO:68378041
Keywords : mesenchymal stem-cells * biophysical regulation * nuclear-structure
Subject RIV: FP - Other Medical Disciplines
Impact factor: 3.252, year: 2016 ; AIS: 1.141, rok: 2016
DOI: https://doi.org/10.1039/c6ib00125d

Intracellular and extracellular mechanical forces play a crucial role during tissue growth, modulating nuclear shape and function and resulting in complex collective cell behaviour. However, the mechanistic understanding of how the orientation, shape, symmetry and homogeneity of cells are affected by environmental geometry is still lacking. Here we investigate cooperative cell behaviour and patterns under geometric constraints created by topographically patterned substrates. We show how cells cooperatively adopt their geometry, shape, positioning of the nucleus and subsequent proliferation activity. Our findings indicate that geometric constraints induce significant squeezing of cells and nuclei, cytoskeleton reorganization, drastic condensation of chromatin resulting in a change in the cell proliferation rate and the anisotropic growth of cultures. Altogether, this work not only demonstrates complex non-trivial collective cellular responses to geometrical constraints but also provides a tentative explanation of the observed cell culture patterns grown on different topographically patterned substrates. These findings provide important fundamental knowledge, which could serve as a basis for better controlled tissue growth and cell-engineering applications.
Permanent Link: http://hdl.handle.net/11104/0268831