Počet záznamů: 1
CyanoP is Involved in the Early Steps of Photosystem II Assembly in the Cyanobacterium Synechocystis sp PCC 6803
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SYSNO ASEP 0470774 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název CyanoP is Involved in the Early Steps of Photosystem II Assembly in the Cyanobacterium Synechocystis sp PCC 6803 Tvůrce(i) Knoppová, Jana (MBU-M) RID, ORCID
Yu, J. (GB)
Koník, P. (CZ)
Nixon, P. (GB)
Komenda, Josef (MBU-M) RID, ORCIDZdroj.dok. Plant and Cell Physiology. - : Oxford University Press - ISSN 0032-0781
Roč. 57, č. 9 (2016), s. 1921-1931Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. JP - Japonsko Klíč. slova Cyanobacteria ; CyanoP ; Photosynthesis Vědní obor RIV EE - Mikrobiologie, virologie CEP GBP501/12/G055 GA ČR - Grantová agentura ČR LM2015055 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000384717400011 EID SCOPUS 84988384578 DOI https://doi.org/10.1093/pcp/pcw115 Anotace Although the PSII complex is highly conserved in cyanobacteria and chloroplasts, the PsbU and PsbV subunits stabilizing the oxygen-evolving Mn4CaO5 cluster in cyanobacteria are absent in chloroplasts and have been replaced by the PsbP and PsbQ subunits. There is, however, a distant cyanobacterial homolog of PsbP, termed CyanoP, of unknown function. Here we show that CyanoP plays a role in the early stages of PSII biogenesis in Synechocystis sp. PCC 6803. CyanoP is present in the PSII reaction center assembly complex (RCII) lacking both the CP47 and CP43 modules and binds to the smaller D2 module. A small amount of larger PSII core complexes co-purifying with FLAG-tagged CyanoP indicates that CyanoP can accompany PSII on most of its assembly pathway. A role in biogenesis is supported by the accumulation of unassembled D1 precursor and impaired formation of RCII in a mutant lacking CyanoP. Interestingly, the pull-down preparations of CyanoP-FLAG from a strain lacking CP47 also contained PsbO, indicating engagement of this protein with PSII at a much earlier stage in assembly than previously assumed. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
Počet záznamů: 1