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Antibody repertoire development in fetal and neonatal piglets. XXIV. Hypothesis: The ileal Peyer patches (IPP) are the major source of primary, undiversified IgA antibodies in newborn piglets
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SYSNO ASEP 0467813 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Antibody repertoire development in fetal and neonatal piglets. XXIV. Hypothesis: The ileal Peyer patches (IPP) are the major source of primary, undiversified IgA antibodies in newborn piglets Tvůrce(i) Butler, J. E. (US)
Santiago-Mateo, K. (US)
Wertz, N. (US)
Sun, X. (CN)
Šinkora, Marek (MBU-M) RID, ORCID
Francis, D. (US)Zdroj.dok. Developmental and Comparative Immunology. - : Elsevier - ISSN 0145-305X
Roč. 65, December (2016), s. 340-351Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Germfree animals ; Ileal Peyers patches ; Immunoglobulin A Vědní obor RIV EC - Imunologie CEP GA15-02274S GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000384388400038 EID SCOPUS 84982243068 DOI https://doi.org/10.1016/j.dci.2016.07.020 Anotace The Heal Peyers patches (IPP) of newborn germfree (GF) piglets were isolated into blind loops and the piglets colonized with a defined probiotic microflora. After 5 weeks, IgA levels in the intestinal lavage (IL) of loop piglets remained at GF levels and IgM comprised similar to 70% while in controls, IgA levels were elevated 5-fold and comprised similar to 70% of total Igs. Loop piglets also had. reduced serum IgA levels suggesting the source of serum IgA had been interrupted. The isotype profile for loop contents was intermediate between that in the IL of GF and probiotic controls. Surprisingly, colonization alone did not result in repertoire diversification in the IPP. Rather, colonization promoted pronounced proliferation of fully switched IgA(+)IgM(-) B cells in the IPP that supply early, non-diversified "natural" SIgA antibodies to the gut lumen and a primary IgA response in serum. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
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