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Isoflavone synthase genes in legumes and non-leguminous plants
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SYSNO ASEP 0440719 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Isoflavone synthase genes in legumes and non-leguminous plants Tvůrce(i) Pičmanová, Martina (UEB-Q)
Koblovská, R. (CZ)
Lapčík, O. (CZ)
Honys, David (UEB-Q) RID, ORCIDZdroj.dok. Proceeding of International Conference on Biomedical Engineering and Biotechnology. - Washington, D.C : IEEE Computer Society, 2012 / Sloan K. - ISBN 978-0-7695-4706-0 Rozsah stran s. 344-347 Poč.str. 4 s. Forma vydání Tištěná - P Akce International Conference on Biomedical Engineering and Biotechnology /2012/ Datum konání 28.05.2012-30.05.2012 Místo konání Macau Země CN - Čína Typ akce WRD Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova legumes ; non-leguminous plants ; isoflavone synthase Vědní obor RIV EF - Botanika CEP GA525/09/0994 GA ČR - Grantová agentura ČR GAP501/11/1462 GA ČR - Grantová agentura ČR OC10054 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 EID SCOPUS 84866726328 DOI https://doi.org/10.1109/iCBEB.2012.257 Anotace Amidst the vast number of diverse secondary metabolites of plants, isoflavonoids occupy a special place due to the wide range of their biological activities. The literature deals extensively with the positive effect of these well-known phytoestrogens on human health, including cancer prevention and the mitigation of menopause symptoms, as well as with the potential risks associated with their consumption. Isoflavonoids have considerable importance for plants themselves, particularly in the defense against pathogens and in the induction of rhizobial symbiosis. A complete description of biosynthetic pathway of these natural products and of the genetic background of this biosynthesis, constitutes a challenge in the metabolic engineering of isoflavonoid biosynthesis, especially in the case of crop-plants that are not natural. Isoflavone synthase plays a key role in the biosynthesis of isoflavonoids. The vast majority of over 1600 known isoflavone structures were found in legumes. However, isoflavonoids have been identified in many other species from over 60 families. Known IFS genes belong to the CYP93C subfamily of cytochrome P450. Due to very specific nature of the catalyzed reaction, we hypothesised that IFS genes in other species share high degree of homology with already cloned genes. We aimed to identify new IFS orthologues in non-leguminous isoflavoneproducing plant species: Cannabis sativa and Humulus lupulus (Cannabaceae), Ruta montana and Ruta graveolens (Rutaceae) and Nicotiana tabacum (Solanaceae). IFS sequences showed high degree of similarity, over 70% at the nucleotide level. The phylogenetic map demonstrates the evolutionary relation of IFS genes from congenial species and their relationship to other taxa. Non-leguminous isoflavone producers contain close IFS orthologues that are likely to catalyze the first step of isoflavone biosynthesis. This finding has the potential to be exploited for the control of isoflavone production- in crop plants. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2015
Počet záznamů: 1