Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface

Paleček, Emil; Černocká, Hana; Ostatná, Veronika; Navrátilová, Lucie; Brázdová, Marie

doi:https://dx.doi.org/10.1016/j.aca.2014.03.029

Počet záznamů: 1

Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface

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SYSNO ASEP	0435478
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Electrochemical sensing of tumor suppressor protein p53-deoxyribonucleic acid complex stability at an electrified interface
Author(s)	Paleček, Emil (BFU-R)_{RID, ORCID} Černocká, Hana (BFU-R)_{RID, ORCID} Ostatná, Veronika (BFU-R)_{RID, ORCID} Navrátilová, Lucie (BFU-R) Brázdová, Marie (BFU-R)_{RID, ORCID}
Number of authors	5
Source Title	Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670 Roč. 828, MAY2014 (2014), s. 1-8
Number of pages	9 s.
Publication form	Print - P
Language	eng - English
Country	NL - Netherlands
Keywords	Deoxyribonucleic acid-protein binding ; Tumor suppressor protein p53 ; Electrochemical sensing
Subject RIV	BO - Biophysics
R&D Projects	GAP301/11/2055 GA ČR - Czech Science Foundation (CSF)
	GA13-00956S GA ČR - Czech Science Foundation (CSF)
	GA13-36108S GA ČR - Czech Science Foundation (CSF)
Institutional support	BFU-R - RVO:68081707
UT WOS	000336336900001
DOI	https://doi.org/10.1016/j.aca.2014.03.029
Annotation	Electrochemical biosensors have the unique ability to convert biological events directly into electrical signals suitable for parallel analysis. Here we utilize specific properties of constant current chronopotentiometric stripping (CPS) in the analysis of protein and DNA-protein complex nanolayers. Rapid potential changes at high negative current intensities (I-str) in CPS are utilized in the analysis of DNA-protein interactions at thiol-modified mercury electrodes. P53 core domain (p53CD) sequence-specific binding to DNA results in a striking decrease in the electrocatalytic signal of free p53. This decrease is related to changes in the accessibility of the electroactive amino acid residues in the p53CD-DNA complex. By adjusting I-str and temperature, weaker non-specific binding can be eliminated or distinguished from the sequence-specific binding. The method also reflects differences in the stabilities of different sequence-specific complexes, including those containing spacers between half-sites of the DNA consensus sequence.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2015

Počet záznamů: 1