0424665 - ÚIACH 2014 CZ eng D - Dizertace
Duša, Filip
Multidimensional separation methods in analysis of complex biological mixtures.
Ústav analytické chemie AV ČR, v. v. i. Obhájeno: Brno. 29.01.2014. - Brno: Masaryk university, Faculty of Science, 2014. 92 s.
Grant CEP: GA MŠMT(CZ) EE2.3.20.0182
Institucionální podpora: RVO:68081715
Klíčová slova: isoelectric focusing * liquid chromatography * two-dimensional separations * sample pretreatment * proteins
Kód oboru RIV: CB - Analytická chemie, separace

This work deals with two-dimensional separations of peptides and proteins from biological matrices. Theoretical introduction describes extraction of proteins from biological matrices, modes of isoelectric focusing (IEF) and liquid chromatography (LC) used for fractionation in two-dimensional separation schemes, and current papers dealing with combination of IEF and LC. In the results chapter there are four papers, which passed reviews and have been published in international scientific journals.
Trvalý link: http://hdl.handle.net/11104/0230708