Počet záznamů: 1
Structural analysis of an eIF3 subcomplex reveals conserved interactions required for a stable and proper translation pre-initiation complex assembly
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SYSNO ASEP 0379099 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Structural analysis of an eIF3 subcomplex reveals conserved interactions required for a stable and proper translation pre-initiation complex assembly Tvůrce(i) Herrmannová, Anna (MBU-M) RID, ORCID
Daujotyte, D. (GB)
Yang, J. - Ch. (GB)
Cuchalová, Lucie (MBU-M)
Gorrec, F. (GB)
Wagner, Susan (MBU-M) RID, ORCID
Dányi, István (MBU-M)
Lukavsky, P. J. (GB)
Valášek, Leoš Shivaya (MBU-M) RID, ORCIDZdroj.dok. Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 40, č. 5 (2012), s. 2294-2311Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova START CODON SELECTION ; RNA RECOGNITION MOTIF ; GCN4 MESSENGER-RNA Vědní obor RIV CE - Biochemie CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000302019900041 DOI https://doi.org/10.1093/nar/gkr765 Anotace Translation initiation factor eIF3 acts as the key orchestrator of the canonical initiation pathway in eukaryotes, yet its structure is greatly unexplored. We report the 2.2 A resolution crystal structure of the complex between the yeast seven-bladed beta-propeller eIF3i/TIF34 and a C-terminal alpha-helix of eIF3b/PRT1, which reveals universally conserved interactions. Mutating these interactions displays severe growth defects and eliminates association of eIF3i/TIF34 and strikingly also eIF3g/TIF35 with eIF3 and 40S subunits in vivo. Unexpectedly, 40S-association of the remaining eIF3 subcomplex and eIF5 is likewise destabilized resulting in formation of aberrant pre-initiation complexes (PICs) containing eIF2 and eIF1, which critically compromises scanning arrest on mRNA at its AUG start codon suggesting that the contacts between mRNA and ribosomal decoding site are impaired Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2013
Počet záznamů: 1