Počet záznamů: 1
The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase
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SYSNO ASEP 0355844 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase Tvůrce(i) Kulik, Natallia (UEK-B) RID
Weignerová, L. (CZ)
Filipi, Tomáš (MBU-M)
Pompach, Petr (MBU-M) RID, ORCID
Novák, Petr (UTIA-B)
Mrázek, H. (CZ)
Slámová, Kristýna (MBU-M) RID, ORCID
Bezouška, K. (CZ)
Křen, Vladimír (MBU-M) RID, ORCID
Ettrich, Rüdiger (UEK-B) RID, ORCID, SAICelkový počet autorů 10 Zdroj.dok. Glycobiology. - : Oxford University Press - ISSN 0959-6658
Roč. 20, č. 11 (2010), s. 1410-1419Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova α-N-acetylgalactosaminidase ; α-galactosidase ; Aspergillus niger ; substrate binding ; molecular modeling Vědní obor RIV CE - Biochemie CEP LC06010 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GAP207/10/1934 GA ČR - Grantová agentura ČR GAP207/10/1040 GA ČR - Grantová agentura ČR GA303/09/0477 GA ČR - Grantová agentura ČR GAP207/10/0321 GA ČR - Grantová agentura ČR CEZ AV0Z60870520 - UEK-B (2005-2011) AV0Z50200510 - MBU-M (2005-2011) AV0Z10750506 - UTIA-B (2005-2011) UT WOS 000282750300009 DOI 10.1093/glycob/cwq105 Anotace Two genes in the genome of Aspergillus niger, aglA and aglB, have been assigned to encode for α-d-galactosidases variant A and B. However, analyses of primary and 3D structures based on structural models of these two enzymes revealed significant differences in their active centers suggesting important differences in their specificity for the hydrolyzed carbohydrates. To test this unexpected finding, a large screening of libraries from 42 strains of filamentous fungi succeeded in identifying an enzyme from A. niger CCIM K2 that exhibited both α-galactosidase andα-N-acetylgalactosaminidase activities, with the latter activity predominating. The enzyme protein was sequenced, and its amino acid sequence could be unequivocally assigned to the enzyme encoded the aglA gene. Enzyme activity measurements and substrate docking clearly demonstrated the preference of the identified enzyme for α-N-acetyl-d-galactosaminide over α-d-galactoside. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2011
Počet záznamů: 1