Počet záznamů: 1
Interaction of plant amine oxidases with diaminoethers
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SYSNO ASEP 0305982 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Interaction of plant amine oxidases with diaminoethers Překlad názvu Interaction of plant amine oxidases with diaminoethers Tvůrce(i) Šebela, M. (CZ)
Jarkovská, K. (CZ)
Lenobel, René (UEB-Q) ORCID
Medda, R. (IT)
Padiglia, A. (IT)
Floris, G. (IT)
Peč, P. (CZ)Zdroj.dok. Arkivoc - ISSN 1424-6376
Part 7, - (2007), s. 222-232Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova diamine oxidase ; diaminoether ; inhibition Vědní obor RIV CE - Biochemie CEZ AV0Z50380511 - UEB-Q (2005-2011) Anotace Polyamines are ubiquitous compounds, which are involved in crucial physiological events including cell growth and differentiation. The catabolic oxidative degradation of polyamines is catalyzed by quinoprotein copper-containing amine oxidases (CAOs) and flavoprotein polyamine oxidases (PAOs). Various synthetic polyamine analogs and polyamine derivatives have been reported, which represent important tools (substrates or inhibitors) in the study of catalytic properties of the enzymes. In this work, two related compounds were studied in the reactions with plant amine oxidases: 1,8-diamino-3,6-dioxaoctane (DADO) and 1,10-bis(2pyridinylmethyl)-4,7-dioxa-1,10-diazadecane (BPDD). Based on activity and stoichiometry assays together with spectrophotometric measurements, DADO can be considered a good substrate for grass pea, lentil and E. characias CAOs with K-m values in the range 10(-4) - 10(-3) M. Its oxidative degradation produces the corresponding aminoaldehyde 1,8-amino-3,6-dioxaoctanal,which does not undergo spontaneous cyclization ( as it is known for the oxidation products of natural substrates putrescine, cadaverine and spermidine) or polymerization in the reaction mixture. Conversely, oat PAO does not oxidize DADO and is only weakly inhibited by the compound (K-i = 1.6 mM towards putrescine). BPDD was found to be a competitive inhibitor of both CAOs and PAOs with Ki values of 10(-4) M. DADO could be suggested as a potential affinity ligand for CAOs. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2008
Počet záznamů: 1