Počet záznamů: 1
Intracellular retention of iodine-125 and astatine-211 labelled epidermal growth factor (EGF) in cultured A431 carcinoma cells
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SYSNO ASEP 0185194 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Intracellular retention of iodine-125 and astatine-211 labelled epidermal growth factor (EGF) in cultured A431 carcinoma cells Tvůrce(i) Orlova, A. (SE)
Sjöström, A. (SE)
Lebeda, Ondřej (UJF-V) RID, ORCID, SAI
Lundquist, H. (SE)
Tolmachev, V. (SE)Zdroj.dok. Nuclear Medicine Communications - ISSN 0143-3636
Roč. 21, - (2000), s. 577Poč.str. 1 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Vědní obor RIV BG - Jaderná, atomová a mol. fyzika, urychlovače CEZ AV0Z1048901 - UJF-V Anotace The fact, that many tumours over-express receptors to epidermal growth factor (EGF), has led us to considered this molecule in targeted radionuclide therapy. In this study different radiohalogens and labelling methods were applied, the binding pattern and the intracellular retention in carcinoma A431 cells of .sup.125I-EGF, .sup.125I-para-iodo-benzoate-EGF (.sup.125I-PIB-EGF), and .sup.211 At-para-astato-benzoate-EGF (.sup.211At-PAB-EGF), were studied. All labelled molecules bound specifically to the A431 cells. The intracellular retention of radioactivity, after pulse labelling, was found to be about the same for the two iodine labels with biological half-life of 1.5-2h. The astatine label gave a prolonged intracellular retention (biological half-life about 3.5 h) in comparison with radioiodine. Whenthe cells where incubated continuously with the labelled ligands, maximum accumulation was obtained later for the astatine label (about 6h) than for both iodine labels (2-4h).=Since the maximum accumulation is reached when the rate ofbinding is equal to the rate of efflux of degraded conjugate, the shift of the maximum position indicates that the excretion of astatine is slower than iodine labels. In conclusion, the use of non-phenolic linker did not improve intracellular retention of radioactivity. The improved intracellular retention of the astatine might be attributed to binding of radioactive catabolites to cellular proteins. Pracoviště Ústav jaderné fyziky Kontakt Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Rok sběru 2001
Počet záznamů: 1