24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA
1.
SYSNO ASEP
0172328
Druh ASEP
J - Článek v odborném periodiku
Zařazení RIV
J - Článek v odborném periodiku
Poddruh J
Ostatní články
Název
24-EPIBRASSINOLIDE AT SUBNANOMOLAR CONCENTRATIONS MODULATES GROWTH AND PRODUCTION CHARACTERISTICS OF A MOUSE HYBRIDOMA
Tvůrce(i)
Franěk, František (UEB-Q) Eckschlager, T. (CZ) Kohout, Ladislav (UOCHB-X)
Zdroj.dok.
Collection of Czechoslovak Chemical Communications. - : Ústav organické chemie a biochemie AV ČR, v. v. i.
- ISSN 0010-0765
Roč. 68, č. 11 (2003), s. 2190-2200
Poč.str.
11 s.
Jazyk dok.
eng - angličtina
Země vyd.
CZ - Česká republika
Klíč. slova
Steroids ; Brassinosteroids ; 24-Epibrassinolides
Vědní obor RIV
CE - Biochemie
CEP
GA203/01/0083 GA ČR - Grantová agentura ČR
OC 844.10 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ
AV0Z5038910 - UEB-Q
MSM 153100008
AV0Z4055905 - UOCHB-X
Anotace
A mouse hybridoma was cultured either in standard serum-free medium, or in medium diluted to 30%, in which the cells underwent nutritional stress. Steady-state parameters of semicontinuous cultures conducted at 24-epibrassinolide concentrations from 10-16 to 10-9 mol l-1 were evaluated. Typical effects of the agent found both in standard and in diluted media were (i) increase in the value of mitochondrial membrane potential, (ii), drop of intracellular antibody level, (iii) increase in the fraction of the cells in the G0/G1 phase, and (iv) decrease in the fraction of the cells in the S phase. Alleviation of nutritional stress manifested itself in cultures conducted in diluted media. At the lowest brassinosteroid concentrations, the number of steroid molecules in the culture was of the same order of magnitude as the number of viable cells in the culture. This implies involvement of a complex cascade mechanism, through which the steroid molecule induces alterations in gene expression leading finally to significant changes in cell culture parameters.
Pracoviště
Ústav experimentální botaniky
Kontakt
David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru
2004
Počet záznamů: 1
openseadragon
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