Influence of the laser power and analyte concentration on the results in MALDI-TOF MS of oligosaccharides
1.
SYSNO ASEP
0162341
Druh ASEP
C - Konferenční příspěvek (mezinárodní konf.)
Zařazení RIV
D - Článek ve sborníku
Název
Influence of the laser power and analyte concentration on the results in MALDI-TOF MS of oligosaccharides
Tvůrce(i)
Čáslavský, Josef (UIACH-O) Chmelík, Josef (UIACH-O)
Zdroj.dok.
Proceedings of the 18th Informal Meeting on Mass Spectrometry. - Prague, 2000
- ISBN 80-238-5344-9
Rozsah stran
s. 26
Poč.str.
1 s.
Akce
Informal meeting on mass spectrometry /18./
Datum konání
30.04.2000-04.05.2000
Místo konání
Praha
Země
CZ - Česká republika
Jazyk dok.
eng - angličtina
Země vyd.
CZ - Česká republika
Klíč. slova
mass spectrometry ; MALDI-TOF ; oligosaccharides
Vědní obor RIV
CB - Analytická chemie, separace
CEZ
AV0Z4031919 - UIACH-O
Anotace
MALDI-TOF MS has been successfully applied for the analysis of a wide range of organic biopolymers including oligosaccharides. We focus our attention to the group of oligosaccharides occuring in beer and in malting and mashing products, which are mainly oligosaccharides containing from 3 to more than 30 glucose units in their molecules. We tried to evaluate various factors influencing the obtained results. We found that the shape of the spectrum depends significantly on the energy of ionising laser pulse and also on the amount of the analyte on the MALDI target. These effects were studied with standard mixtures as well as with real samples.