Optimization of experimental conditions for characterization of barley starch granules by gravitational field-flow fractionation
1.
SYSNO ASEP
0162309
Druh ASEP
C - Konferenční příspěvek (mezinárodní konf.)
Zařazení RIV
D - Článek ve sborníku
Název
Optimization of experimental conditions for characterization of barley starch granules by gravitational field-flow fractionation
Tvůrce(i)
Janoušková, Jana (UIACH-O) Budinská, Marcela (UIACH-O) Plocková, Jana (UIACH-O) Krumlová, Andrea (UIACH-O) Chmelík, Josef (UIACH-O)
Zdroj.dok.
FFF-2001. Ninth International Symposium on Field-Flow Fractionation. - Colorado : Department of Chemistry and Geochemistry, Colorado School of Mines, 2001
Rozsah stran
s. p3
Poč.str.
1 s.
Akce
International Symposium on Field-Flow Fractionation /9./
Datum konání
26.06.2001-29.06.2001
Místo konání
Colorado
Země
US - Spojené státy americké
Typ akce
WRD
Jazyk dok.
eng - angličtina
Země vyd.
US - Spojené státy americké
Klíč. slova
starch ; field-flow fractionation
Vědní obor RIV
CB - Analytická chemie, separace
CEP
IAA4031805 GA AV ČR - Akademie věd
CEZ
AV0Z4031919 - UIACH-O
Anotace
Separation of small and large barley starch granules by gravitational field-flow fractionation (GFFF) was investigated from the point of view of sample pre-treatment, amount of injected sample, and elution conditions. The sample pre-treatment study resulted in the conclusion that it is reasonable to soak the starch granules for at least 24 hours prior to separation. The experiments with different amounts of injected sample show that it is possible to increase twice as well as decrease twice the sample amount usually used without any change in retention ratios. The implementation of flow-rate gradients for elution of the starch granules reduced total separation time. However, the applied flow-rate gradients did not improve the resolution of the peaks A and B compared with the generally used constant flow-rate. Thus, for barley starch granules, the constant flow-rates within the range from 0.8 ml/min to 1.0 ml/min seem to provide the best compromise of total separation time, peak resolution and instrumental expenses.
