0162299 - UIACH-O 20010050 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Horká, Marie - Šlais, Karel
Dynamic modification of proteins for fluorometric detection in CZE.
2nd International Symposium Separations in the BioSciences. SBS 2001. Praha, 2001, s. 91. ISBN 80-7080-437-8.
[International Symposium Separations in the BioSciences /2./. Praha (CZ), 17.09.2001-20.09.2001]
Grant CEP: GA AV ČR IAA4031901
Výzkumný záměr: CEZ:AV0Z4031919
Klíčová slova: capillary zone electrophoresis * fluorometric detection * dynamic modification
Kód oboru RIV: CB - Analytická chemie, separace

The separation techniques employing fluorescence detection are sensitive and selective so they have been often applied for the trace analysis of biological samples. The commonly used derivatization of proteins can lower the detection limits; however, they can change the acido-basic properties and mobilities when compared to the native species. Recently, we have used the colored tenside as a buffer additive for the photometric detection of proteins in the UV region. The selectivity, efficiency and resolution of CZE separation using this dye were found to be similar to the CZE with SDS as the additive. In this study, the ampiphilic fluorescent compound is suggested as a buffer additive in CZE for dynamic modification of the sample of several proteins. Using the deuterium lamp for the excitation in the UV region for the on column fluorometric detection, the amol minimum detectable amounts of the proteins sampled on the CZE capillary were achieved.
Trvalý link: http://hdl.handle.net/11104/0059618