Počet záznamů: 1
NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation
- 1.
SYSNO ASEP 0105350 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation Překlad názvu Fosforylace NTAL je klíčovým spojem v signalizačních kaskádách vedoucím k degranulaci lidských žírných buněk po aktivaci Kit a agregaci Fc epsilon RI Tvůrce(i) Tkaczyk, C. (US)
Hořejší, Václav (UMG-J) RID
Iwaki, S. (US)
Dráber, Petr (UMG-J) RID
Samelson, L. E. (US)
Satterthwaite, A. B. (US)
Nahm, D. H. (US)
Metcalfe, D. D. (US)
Gilfillan, A. M. (US)Zdroj.dok. Blood. - : American Society of Hematology - ISSN 0006-4971
Roč. 104, č. 1 (2004), s. 207-214Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova immunoreceptor ; signalling Vědní obor RIV EC - Imunologie CEP LN00A026 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z5052915 - UMG-J Anotace Aggregation of high-affinity receptors for immunoglobulin E (Fc epsilon RI) on the surface of mast cells results in degranulation, a response that is potentiated by binding of stem cell factor (SCF) to its receptor Kit. We observed that one of the major initial signaling events associated with Fc epsilon RI-mediated activation of human mast cells (HuMCs) is the rapid tyrosine phosphorylation of a protein of 25 to 30 kDa. The phosphorylation of this protein was also observed in response to SCF. This protein was identified as non-T-cell activation linker (NTAL), an adaptor molecule similar to linker for activated T cells (LAT). Unlike the Fc epsilon RI response, SCF induced NTAL phosphorylation in the absence of detectable LAT phosphorylation. When SCF and antigen were added concurrently, there was a marked synergistic effect on NTAL phosphorylation, however, SCF did not enhance the phosphorylation of LAT induced by Fc epsilon RI aggregation. Fc epsilon RI- and SCF-mediated NTAL phosphorylation appear to be differentially regulated by Src kinases and/or Kit kinase, respectively. Diminution of NTAL expression by silencing RNA oligonucleotides in HuMCs resulted in a reduction of both Kit- and Fc epsilon RI-mediated degranulation. NTAL, thus, appears to be an important link between the signaling pathways that are initiated by these receptors, culminating in mast cell degranulation Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2010
Počet záznamů: 1