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Fluorescent poly[N-(2-hydroxypropyl) methacrylamide] nanogel by dispersion polymerization as a contrast agent for live-cell imaging
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SYSNO ASEP 0574199 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fluorescent poly[N-(2-hydroxypropyl) methacrylamide] nanogel by dispersion polymerization as a contrast agent for live-cell imaging Tvůrce(i) Šálek, Petr (UMCH-V) RID, ORCID
Zbořilová, D. (CZ)
Pavlova, Ewa (UMCH-V) RID
Kočková, Olga (UMCH-V) RID, ORCID
Konefal, Rafal (UMCH-V) RID, ORCID
Morávková, Zuzana (UMCH-V) RID, ORCID
Janoušková, O. (CZ)Číslo článku e54331 Zdroj.dok. Journal of Applied Polymer Science. - : Wiley - ISSN 0021-8995
Roč. 140, č. 34 (2023)Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova cell uptake ; dispersion polymerization ; imaging Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 UT WOS 001024203900001 EID SCOPUS 85164310907 DOI 10.1002/app.54331 Anotace Here, we report a novel dispersion polymerization for the preparation of cross-linked poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA)-based nanogels in water/2-methoxyethanol mixture (H2O/MetCel), initiated with potassium persulfate (KPS), and stabilized with poly(vinyl alcohol) 25/140 (PVA) and sodium dodecyl sulfate (SDS). Obtained nanogels were characterized using transmission (TEM) and cryogenic transmission electron microscopy (cryo-TEM), dynamic light scattering (DLS), asymmetric flow field-flow fractionation (A4F), nuclear magnetic resonance spectroscopy (NMR), and Raman spectroscopy methods in terms of size, particle size distribution, morphology, and structure. N-(2-hydroxypropyl) methacrylamide (HPMA) was copolymerized with 20 wt% ethylene dimethacrylate (EDMA) resulting in 138 nm poly[N-(2-hydroxypropyl) methacrylamide-co-ethylene dimethacrylate] (PHPMA-EDMA) nanogel dispersion with irregular shape and core-shell type structure. Next, we copolymerized HPMA with 20 wt% EDMA and 10 wt% propargyl methacrylate (PMA) to incorporate reactive functionality into the final core-shell type 120 nm poly[N-(2-hydroxypropyl) methacrylamide-co-ethylene dimethacrylate-co-propargyl methacrylate] (PHPMA-EDMA-PMA) nanogel dispersion. Then, the biocompatibility of PHPMA-EDMA-PMA nanogel was proved using rat mesenchymal stem cells (rMSC), and human foreskin fibroblasts (BJ). PHPMA-EDMA-PMA nanogel was fluorescently labeled with sulfo-cyanine3 azide resulting in 131 nm nanogel. We performed in vitro uptake studies with fluorescently labeled PHPMA-EDMA-PMA nanogel using rMSC showing that the fluorescently labeled PHPMA-EDMA-PMA nanogel was well-distributed in the cytosol and taken up into lysosomes.
Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2024 Elektronická adresa https://onlinelibrary.wiley.com/doi/10.1002/app.54331
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