Počet záznamů: 1
Genomic survey maps differences in the molecular complement of vesicle formation machinery between iGiardia intestinalis/i assemblages
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SYSNO ASEP 0583491 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Genomic survey maps differences in the molecular complement of vesicle formation machinery between iGiardia intestinalis/i assemblages Tvůrce(i) Pipaliya, S. (CA)
Dacks, Joel Bryan (BC-A) SAI, ORCID
Croxen, M.A. (CA)Celkový počet autorů 3 Číslo článku e0011837 Zdroj.dok. PLoS Neglected Tropical Diseases. - : Public Library of Science - ISSN 1935-2735
Roč. 17, č. 12 (2023)Poč.str. 22 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova endoplasmic-reticulum ; escrt machinery ; lamblia ; duodenalis ; identification ; epidemiology ; neogenesis ; organelle ; subunit ; cell Vědní obor RIV FN - Epidemiologie, infek. nemoci a klin. imunologie Obor OECD Infectious Diseases Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 001153606300002 EID SCOPUS 85181193505 DOI 10.1371/journal.pntd.0011837 Anotace Giardia intestinalis is a globally important microbial pathogen with considerable public health, agricultural, and economic burden. Genome sequencing and comparative analyses have elucidated G. intestinalis to be a taxonomically diverse species consisting of at least eight different sub-types (assemblages A-H) that can infect a great variety of animal hosts, including humans. The best studied of these are assemblages A and B which have a broad host range and have zoonotic transmissibility towards humans where clinical Giardiasis can range from asymptomatic to diarrheal disease. Epidemiological surveys as well as previous molecular investigations have pointed towards critical genomic level differences within numerous molecular pathways and families of parasite virulence factors within assemblage A and B isolates. In this study, we explored the necessary machinery for the formation of vesicles and cargo transport in 89 Canadian isolates of assemblage A and B G. intestinalis. Considerable variability within the molecular complement of the endolysosomal ESCRT protein machinery, adaptor coat protein complexes, and ARF regulatory system have previously been reported. Here, we confirm inter-assemblage, but find no intra-assemblage variation within the trafficking systems examined. This variation includes losses of subunits belonging to the ESCRTIII as well as novel lineage specific duplications in components of the COPII machinery, ARF1, and ARFGEF families (BIG and CYTH). Since differences in disease manifestation between assemblages A and B have been controversially reported, our findings may well have clinical implications and even taxonomic, as the membrane trafficking system underpin parasite survival, pathogenesis, and propagation. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2024 Elektronická adresa https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0011837
Počet záznamů: 1