Počet záznamů: 1
Derivatization in Analytical Chemistry
- 1.
SYSNO ASEP 0558090 Druh ASEP M - Kapitola v monografii Zařazení RIV Záznam nebyl označen do RIV Název Structural Characterization of Unusual Fatty Acid Methyl Esters with Double and Triple Bonds Using HPLC/APCI-MS2 with Acetonitrile In-Source Derivatization Tvůrce(i) Horká, Petra (UOCHB-X)
Vrkoslav, Vladimír (UOCHB-X) RID, ORCID
Kindl, Jiří (UOCHB-X) RID
Schwarzová-Pecková, K. (CZ)
Cvačka, Josef (UOCHB-X) RID, ORCIDZdroj.dok. Derivatization in Analytical Chemistry. - Basel : MDPI, 2022 / Tzanavaras P. D. - ISBN 978-3-0365-4256-0 Rozsah stran s. 39-59 Poč.str. 21 s. Poč.str.knihy 200 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova acetonitrile-related adducts ; acetylenic lipids ; double and triple bond localization ; in-source derivatization ; mass spectrometry Obor OECD Analytical chemistry CEP EF16_019/0000729 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UOCHB-X - RVO:61388963 DOI 10.3390/books978-3-0365-4255-3 Anotace Double and triple bonds have significant effects on the biological activities of lipids. Determining multiple bond positions in their molecules by mass spectrometry usually requires chemical derivatization. This work presents an HPLC/MS method for pinpointing the double and triple bonds in fatty acids. Fatty acid methyl esters were separated by reversed-phase HPLC with an acetonitrile mobile phase. In the APCI source, acetonitrile formed reactive species, which added to double and triple bonds to form [M + C3H5N]+• ions. Their collisional activation in an ion trap provided fragments helpful in localizing the multiple bond positions. This approach was applied to fatty acids with isolated, cumulated, and conjugated double bonds and triple bonds. The fatty acids were isolated from the fat body of early-nesting bumblebee Bombus pratorum and seeds or seed oils of Punicum granatum, Marrubium vulgare, and Santalum album. Using the method, the presence of the known fatty acids was confirmed, and new ones were discovered. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2023
Počet záznamů: 1