Počet záznamů: 1
Bordetella Adenylate Cyclase Toxin Elicits Airway Mucin Secretion through Activation of the cAMP Response Element Binding Protein
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SYSNO ASEP 0547494 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bordetella Adenylate Cyclase Toxin Elicits Airway Mucin Secretion through Activation of the cAMP Response Element Binding Protein Tvůrce(i) Malandra, Anna (MBU-M) ORCID
Rahman, Waheed Ur (MBU-M) ORCID
Klímová, Nela (MBU-M) ORCID
Streparola, Gaia (MBU-M)
Holubová, Jana (MBU-M) RID, ORCID
Osičková, Adriana (MBU-M) RID, ORCID
Bariselli, S. (IT)
Šebo, Peter (MBU-M) RID, ORCID
Osička, Radim (MBU-M) RID, ORCIDČíslo článku 9064 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 16 (2021)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova adenylate cyclase toxin ; Bordetella ; cAMP ; creb ; epithelium ; mucin ; pertussis toxin Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA19-12695S GA ČR - Grantová agentura ČR GX19-27630X GA ČR - Grantová agentura ČR LM2018133 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF18_046/0015861 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CCP II - 90126 - Ústav molekulární genetiky AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000689237100001 EID SCOPUS 85113730131 DOI 10.3390/ijms22169064 Anotace The mucus layer protects airway epithelia from damage by noxious agents. Intriguingly, Bordetella pertussis bacteria provoke massive mucus production by nasopharyngeal epithelia during the initial coryza-like catarrhal stage of human pertussis and the pathogen transmits in mucus-containing aerosol droplets expelled by sneezing and post-nasal drip-triggered cough. We investigated the role of the cAMP-elevating adenylate cyclase (CyaA) and pertussis (PT) toxins in the upregulation of mucin production in B. pertussis-infected airway epithelia. Using human pseudostratified airway epithelial cell layers cultured at air-liquid interface (ALI), we show that purified CyaA and PT toxins (100 ng/mL) can trigger production of the major airway mucins Muc5AC and Muc5B. Upregulation of mucin secretion involved activation of the cAMP response element binding protein (CREB) and was blocked by the 666-15-Calbiochem inhibitor of CREB-mediated gene transcription. Intriguingly, a B. pertussis mutant strain secreting only active PT and producing the enzymatically inactive CyaA-AC(-) toxoid failed to trigger any important mucus production in infected epithelial cell layers in vitro or in vivo in the tracheal epithelia of intranasally infected mice. In contrast, the PT- toxoid-producing B. pertussis mutant secreting the active CyaA toxin elicited a comparable mucin production as infection of epithelial cell layers or tracheal epithelia of infected mice by the wild-type B. pertussis secreting both PT and CyaA toxins. Hence, the cAMP-elevating activity of B. pertussis-secreted CyaA was alone sufficient for activation of mucin production through a CREB-dependent mechanism in B. pertussis-infected airway epithelia in vivo. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/1422-0067/22/16/9064
Počet záznamů: 1