Počet záznamů: 1
G-quadruplexes in H1N1 influenza genomes
- 1.
SYSNO ASEP 0541909 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název G-quadruplexes in H1N1 influenza genomes Tvůrce(i) Brázda, Václav (BFU-R) RID, ORCID
Porubiakova, Otilia (BFU-R)
Cantara, Alessio (BFU-R)
Bohalova, Natalia (BFU-R) ORCID
Coufal, Jan (BFU-R) ORCID
Bartas, Martin (BFU-R)
Fojta, Miroslav (BFU-R) RID, ORCID
Mergny, Jean-Louis (BFU-R) ORCID, RIDCelkový počet autorů 8 Číslo článku 77 Zdroj.dok. BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 22, č. 1 (2021)Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Influenza virus ; G-quadruplex ; G4Hunter Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP EF15_003/0000477 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000613379300001 EID SCOPUS 85099932787 DOI 10.1186/s12864-021-07377-9 Anotace BackgroundInfluenza viruses are dangerous pathogens. Seventy-Seven genomes of recently emerged genotype 4 reassortant Eurasian avian-like H1N1 virus (G4-EA-H1N1) are currently available. We investigated the presence and variation of potential G-quadruplex forming sequences (PQS), which can serve as targets for antiviral treatment.ResultsPQS were identified in all 77 genomes. The total number of PQS in G4-EA-H1N1 genomes was 571. Interestingly, the number of PQS per genome in individual close relative viruses varied from 4 to 12. PQS were not randomly distributed in the 8 segments of the G4-EA-H1N1 genome, the highest frequency of PQS being found in the NP segment (1.39 per 1000nt), which is considered a potential target for antiviral therapy. In contrast, no PQS was found in the NS segment. Analyses of variability pointed the importance of some PQS, even if genome variation of influenza virus is extreme, the PQS with the highest G4Hunter score is the most conserved in all tested genomes. G-quadruplex formation in vitro was experimentally confirmed using spectroscopic methods.ConclusionsThe results presented here hint several G-quadruplex-forming sequences in G4-EA-H1N1 genomes, that could provide good therapeutic targets. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-021-07377-9
Počet záznamů: 1