Počet záznamů: 1
Condensation reactions catalyzed by α-N-acetylgalactosaminidase from Aspergillus niger yielding a-N-acetylgalactosaminides
- 1.0340836 - MBÚ 2010 RIV GB eng J - Článek v odborném periodiku
Weignerová, Lenka - Pelantová, Helena - Manglová, Dana - Michálková, Klára - Křen, Vladimír
Condensation reactions catalyzed by α-N-acetylgalactosaminidase from Aspergillus niger yielding a-N-acetylgalactosaminides.
Biocatalysis and Biotransformation. Roč. 28, č. 2 (2010), s. 150-155. ISSN 1024-2422. E-ISSN 1029-2446
Grant CEP: GA MŠMT OC 136
Klíčová slova: alpha-N-Acetylgalactosaminidase * amino acid glycosylation * enzymatic glycosylation
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 1.275, rok: 2010
Extracellular a-N-acetylgalactosaminidase from Aspergillus niger catalyzed glycosylation yielding a series of 2-acetamido-2-deoxy-a-D-galactobiosides using 2-acetamido-2-deoxy-D-galactopyranose as a glycosyl donor. The isomers a-D-GalpNAc-D-GalpNAc, a-D-GalpNAc-D-GalpNAc and a-D-GalpNAc-D-GalfNAc were isolated and spectrally characterized. The purified enzyme was further used for the glycosylation of free amino acids (serine and threonine) and their N-(tert-butoxycarbonyl)-protected analogs to synthesize the Tn antigen (GalpNAc-a-O-Ser/Thr) and its N-(tertbutoxycarbonyl)-protected derivatives
Trvalý link: http://hdl.handle.net/11104/0183998
Počet záznamů: 1