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Sigma regulatory network in Rhodococcus erythropolis CCM2595
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SYSNO ASEP 0555955 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Sigma regulatory network in Rhodococcus erythropolis CCM2595 Tvůrce(i) Štěpánek, Václav (MBU-M) RID, ORCID
Dostálová, Hana (MBU-M)
Busche, T. (DE)
Blumenstein, Jan (MBU-M)
Grulich, Michal (MBU-M) RID
Plašil, Lukáš (MBU-M)
Rucká, Lenka (MBU-M)
Nešvera, Jan (MBU-M) RID
Pátek, Miroslav (MBU-M) RID, ORCIDČíslo článku fnac014 Zdroj.dok. FEMS Microbiology Letters. - : Oxford University Press - ISSN 0378-1097
Roč. 369, č. 1 (2022)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova corynebacterium-glutamicum promoters ; vitro transcription system ; in-vitro ; environmental-stress ; expression ; responses ; tools ; vivo ; Rhodococcus erythropolis ; transcriptional regulatory network ; sigma factor ; in vitro transcription ; RNA-seq Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA18-13254S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000764887700002 EID SCOPUS 85125019957 DOI 10.1093/femsle/fnac014 Anotace The aim of this investigation was to discover the promoters that drive expression of the sig genes encoding sigma factors of RNA polymerase in Rhodococcus erythropolis CCM2595 and classify these promoters according to the sigma factors which control their activity. To analyze the regulation of major sigma factors, which control large regulons that also contain genes expressed under exponential growth and non-stressed conditions, we used the R. erythropolis CCM2595 culture, which grew rapidly in minimal medium. The transcriptional start sites (TSSs) of the genes sigA, sigB, sigD, sigE, sigG, sigH, sigJ, and sigK were detected by primary 5 '-end-specific RNA sequencing. The promoters localized upstream of the detected TSSs were defined by their35 and10 elements, which were identical or closely similar to these sequences in the related species Corynebacterium glutamicum and Mycobacterium tuberculosis. Regulation of the promoter activities by different sigma factors was demonstrated by two independent techniques (in vivo and in vitro). All analyzed sig genes encoding the sigma factors with extracytoplasmic function (ECF) were found to be also driven from additional housekeeping promoters. Based on the classification of the sig gene promoters, a model of the basic sigma transcriptional regulatory network in R. erythropolis was designed. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://academic.oup.com/femsle/article-abstract/369/1/fnac014/6528914?redirectedFrom=fulltext#no-access-message
Počet záznamů: 1