Počet záznamů: 1
Proportions of taxa belonging to the gut core microbiome change throughout the life cycle and season of the bark beetle Ips typographus
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SYSNO ASEP 0578266 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Proportions of taxa belonging to the gut core microbiome change throughout the life cycle and season of the bark beetle Ips typographus Tvůrce(i) Veselská, Tereza (MBU-M) ORCID
Švec, Karel (MBU-M) ORCID
Kostovčík, Martin (MBU-M) RID, ORCID
Peral-Aranega, E. (ES)
García Fraile, Paula (MBU-M)
Křížková, Barbora (MBU-M)
Havlíček, Václav (MBU-M)
Saati-Santamaria, Zaki (MBU-M) ORCID, RID
Kolařík, Miroslav (MBU-M) RID, ORCIDČíslo článku fiad072 Zdroj.dok. FEMS Microbiology Ecology. - : Oxford University Press - ISSN 0168-6496
Roč. 99, č. 8 (2023)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova dendroctonus-ponderosae coleoptera ; picea-abies ; bacterial communities ; vectored fungi ; diversity ; spruce ; plant ; phytopathogenicity ; visualization ; verbenone ; bark beetles ; core microbiome ; DNA and RNA metabarcoding ; gut fungal and bacterial community ; Ips typographus ; seasonality Obor OECD Microbiology CEP GA19-09072S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 001035142900001 EID SCOPUS 85165521296 DOI 10.1093/femsec/fiad072 Anotace The European spruce bark beetle, Ips typographus, is a serious pest of spruce forests in Europe, and its invasion and development inside spruce tissues are facilitated by microorganisms. We investigated the core gut bacterial and fungal microbiomes of I. typographus throughout its life cycle in spring and summer generations. We used cultivation techniques and molecular identification in combination with DNA and RNA metabarcoding. Our results revealed that communities differ throughout their life cycle and across generations in proportion of dominantly associated microbes, rather than changes in species composition. The bacteriome consisted mostly of the phylum Gammaproteobacteria, with the most common orders and genera being Enterobacteriales (Erwinia and Serratia), Pseudomonadales (Pseudomonas), and Xanthomonadales. The fungal microbiome was dominated by yeasts (Saccharomycetes-Wickerhamomyces, Kuraishia, and Nakazawaea), followed by Sordariomycetes (Ophiostoma bicolor and Endoconidiophora polonica). We did not observe any structure ensuring long-term persistence of microbiota on any part of the gut epithelium, suggesting that microbial cells are more likely to pass through the beetle's gut with chyme. The most abundant taxa in the beetle's gut were also identified as dominant in intact spruce phloem. Therefore, we propose that these taxa are acquired from the environment rather than specifically vectored between generations. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2024 Elektronická adresa https://academic.oup.com/femsec/article/99/8/fiad072/7209148?login=true
Počet záznamů: 1