Počet záznamů: 1
Reduction-dependent siderophore assimilation in a model pennate diatom
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SYSNO ASEP 0518853 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Reduction-dependent siderophore assimilation in a model pennate diatom Tvůrce(i) Coale, T.H. (US)
Moosburner, M. (US)
Horák, Aleš (BC-A) RID, ORCID
Oborník, Miroslav (BC-A) RID, ORCID
Barbeau, K. A. (US)
Allen, A. E. (US)Celkový počet autorů 6 Zdroj.dok. Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 116, č. 47 (2019), s. 23609-23617Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova iron-binding ligands ; phaeodactylum-tricornutum ; amphiphilic siderophores ; thalassiosira-oceanica ; ferric reductase ; organic-ligands ; marine ; transport ; pacific ; acquisition ; diatom ; iron acquisition ; ferric reductase ; siderophore ; phytoplankton Vědní obor RIV EA - Morfologické obory a cytologie Obor OECD Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology CEP GA18-13458S GA ČR - Grantová agentura ČR EF16_019/0000759 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000498683000040 EID SCOPUS 85075228946 DOI 10.1073/pnas.1907234116 Anotace Iron uptake by diatoms is a biochemical process with global biogeochemical implications. In large regions of the surface ocean diatoms are both responsible for the majority of primary production and frequently experiencing iron limitation of growth. The strategies used by these phytoplankton to extract iron from seawater constrain carbon flux into higher trophic levels and sequestration into sediments. In this study we use reverse genetic techniques to target putative iron-acquisition genes in the model pennate diatom Phaeodactylum tricornutum. We describe components of a reduction-dependent siderophore acquisition pathway that relies on a bacterial-derived receptor protein and provides a viable alternative to inorganic iron uptake under certain conditions. This form of iron uptake entails a close association between diatoms and siderophore-producing organisms during low-iron conditions. Homologs of these proteins are found distributed across diatom lineages, suggesting the significance of siderophore utilization by diatoms in the marine environment. Evaluation of specific proteins enables us to confirm independent iron-acquisition pathways in diatoms and characterize their preferred substrates. These findings refine our mechanistic understanding of the multiple iron-uptake systems used by diatoms and help us better predict the influence of iron speciation on taxa-specific iron bioavailability. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2020 Elektronická adresa https://www.pnas.org/content/pnas/116/47/23609.full.pdf
Počet záznamů: 1