Počet záznamů: 1
Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells
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SYSNO ASEP 0555445 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells Tvůrce(i) Hofmanová, Jiřina (BFU-R) RID, ORCID
Slavík, J. (CZ)
Ciganek, M. (CZ)
Ovesna, Petra (BFU-R) ORCID
Tylichová, Zuzana (BFU-R) ORCID
Karasová, Martina (BFU-R) ORCID
Zapletal, Ondřej (BFU-R) ORCID
Straková, Nicol (BFU-R) RID, ORCID
Procházková, Jiřina (BFU-R) RID, ORCID
Bouchal, J. (CZ)
Kolář, Z. (CZ)
Ehrmann, J. (CZ)
Levková, M. (CZ)
Huškova, Z. (CZ)
Skalicky, P. (CZ)
Kozubík, Alois (BFU-R) RID, ORCID
Machala, M. (CZ)
Vondráček, Jan (BFU-R) RID, ORCIDCelkový počet autorů 18 Číslo článku 6650 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI - ISSN 1661-6596
Roč. 22, č. 13 (2021)Poč.str. 16 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova stearoyl-coa desaturase-1 ; atp-citrate lyase ; colorectal-cancer ; lipidomics ; expression ; synthase Obor OECD Biochemistry and molecular biology CEP NV15-30585A GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000671202800001 EID SCOPUS 85108198164 DOI https://doi.org/10.3390/ijms22136650 Anotace The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM(+) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/1422-0067/22/13/6650
Počet záznamů: 1