Počet záznamů: 1  

Lung Tissue Delivery of Virus-Like Particles Mediated by Macrolide Antibiotics

  1. 1.
    SYSNO ASEP0507773
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevLung Tissue Delivery of Virus-Like Particles Mediated by Macrolide Antibiotics
    Tvůrce(i) Crooke, S. N. (US)
    Schimer, Jiří (UOCHB-X) RID, ORCID
    Raji, I. (US)
    Wu, B. (US)
    Oyelere, A. K. (US)
    Finn, M. G. (US)
    Zdroj.dok.Molecular Pharmaceutics. - : American Chemical Society - ISSN 1543-8384
    Roč. 16, č. 7 (2019), s. 2947-2955
    Poč.str.9 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovamacrophage ; macrolide ; virus-like particles ; azithromycin ; clarithromycin ; lung targeting
    Vědní obor RIVCE - Biochemie
    Obor OECDBiochemistry and molecular biology
    Způsob publikováníOmezený přístup
    Institucionální podporaUOCHB-X - RVO:61388963
    UT WOS000474475400011
    EID SCOPUS85068190698
    DOI10.1021/acs.molpharmaceut.9b00180
    AnotaceMacrophage cells are present in high abundance in the lung to intercept invading microorganisms that gain access through airway mucosal surfaces. Several bacterial pathogens have evolved the capacity to evade the innate immune response by establishing infections within pulmonary macrophages upon phagocytosis, leading to prolonged disease. Macrolide antibiotics such as azithromycin and clarithromycin accumulate in phagocytic cells and have been shown to preferentially distribute in tissues where populations of these cells reside. We employed this class of molecules as targeting ligands to direct virus-like particles (VLPs) to lung-resident macrophages. VLP-macrolide conjugates showed enhanced uptake into RAW 264.7 macrophage cells in culture, with azithromycin displaying the greatest effect, distinct differences were also observed for different macrocycle structures and orientations on the particle surface. Activation of macrophage cells was stimulated by particle uptake toward an intermediate activation state, in contrast to previous reports using macrolide-functionalized gold nanorods that stimulated a cytotoxic macrophage response. Attached azithromycin was also able to direct VLPs to the lungs in mice, with significant accumulation within 2 h of systemic injection. These results suggest that this new class of bioconjugate could serve as an effective platform for intracellular drug delivery in the context of pulmonary infections.
    PracovištěÚstav organické chemie a biochemie
    Kontaktasep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
    Rok sběru2020
    Elektronická adresahttps://pubs.acs.org/doi/10.1021/acs.molpharmaceut.9b00180
Počet záznamů: 1  

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