Počet záznamů: 1
Coupling Stable Isotope Labeling and Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight-Tandem Mass Spectrometry for De Novo Mosquito Ovarian Lipid Studies
- 1.0557411 - BC 2023 RIV US eng J - Článek v odborném periodiku
Tose, L. - Ramirez, C. - Michalkova, V. - Nouzová, Marcela - Noriega, F. G. - Fernandez-Lima, F.
Coupling Stable Isotope Labeling and Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight-Tandem Mass Spectrometry for De Novo Mosquito Ovarian Lipid Studies.
Analytical Chemistry. Roč. 94, č. 16 (2022), s. 6139-6145. ISSN 0003-2700. E-ISSN 1520-6882
Institucionální podpora: RVO:60077344
Klíčová slova: aedes-aegypti * juvenile-hormone * fat-body * reserves * acids * bioanalysis * maturation * tracers * c-13
Obor OECD: Zoology
Impakt faktor: 7.4, rok: 2022
Způsob publikování: Omezený přístup
https://pubs.acs.org/doi/10.1021/acs.analchem.1c05090
There is a need to better understand lipid metabolism during mosquito ovarian development. Lipids are the major source of energy supporting ovarian follicles development in mosquitoes. In this paper, we describe the complementary use of stable isotope labeling (SIL) and high-resolution mass spectrometry-based tools for the investigation of de now triglycerides (TG) and diglycerides (DG) during the ovarian previtellogenic (PVG) stage (4-6 days posteclosion) of female adult Aedes aegypti. Liquid chromatography coupled to high-resolution trapped ion mobility spectrometry-parallel accumulation sequential fragmentation-time-of-flight tandem mass spectrometry (LC-TIMS-PASEF-TOF MS/MS) allowed the separation and quantification of nonlabeled and H-1/C-13-labeled TG and DG species. Three SIL strategies were evaluated (H2O/(H2O)-H-2 with 50:50 and 95:5 mixtures, C-13-sucrose, and C-13-glucose). Results showed wide applicability with no signs of lipid ovarian impairment by SIL induced toxicity. The analytical workflow based on LC-TIMS-TOF MS/MS provided high confidence and high reproducibility for lipid DG and TG identification and SIL incorporation based on their separation by retention time (RT), collision cross section (CCS), and accurate m/z. In addition, the SIL fatty acid chain incorporation was evaluated using PASEF MS/MS. The H-2/C-13 incorporation into the mosquito diet provided information on how TG lipids are consumed, stored, and recycled during the PVG stage of ovarian development.
Trvalý link: https://hdl.handle.net/11104/0340420
Počet záznamů: 1