Počet záznamů: 1  

Gradual Response of Cyanobacterial Thylakoids to Acute High-Light Stress-Importance of Carotenoid Accumulation

  1. 1.
    SYSNO ASEP0546702
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevGradual Response of Cyanobacterial Thylakoids to Acute High-Light Stress-Importance of Carotenoid Accumulation
    Tvůrce(i) Canonico, Myriam (MBU-M) ORCID
    Konert, Grzegorz (MBU-M) ORCID
    Crepin, Aurelia (MBU-M) ORCID
    Šedivá, Barbora (MBU-M)
    Kaňa, Radek (MBU-M) RID, ORCID
    Číslo článku1916
    Zdroj.dok.Cells. - : MDPI
    Roč. 10, č. 8 (2021)
    Poč.str.20 s.
    Jazyk dok.eng - angličtina
    Země vyd.CH - Švýcarsko
    Klíč. slovahigh light ; thylakoid membrane ; microdomains ; carotenoids ; photoprotection ; Synechocystis ; non-photochemical quenching ; photoinhibition ; photosystems
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    CEPGA19-11494S GA ČR - Grantová agentura ČR
    ED2.1.00/19.0392 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOpen access
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000688949100001
    EID SCOPUS85115042828
    DOI10.3390/cells10081916
    AnotaceLight plays an essential role in photosynthesis, however, its excess can cause damage to cellular components. Photosynthetic organisms thus developed a set of photoprotective mechanisms (e.g., non-photochemical quenching, photoinhibition) that can be studied by a classic biochemical and biophysical methods in cell suspension. Here, we combined these bulk methods with single-cell identification of microdomains in thylakoid membrane during high-light (HL) stress. We used Synechocystis sp. PCC 6803 cells with YFP tagged photosystem I. The single-cell data pointed to a three-phase response of cells to acute HL stress. We defined: (1) fast response phase (0-30 min), (2) intermediate phase (30-120 min), and (3) slow acclimation phase (120-360 min). During the first phase, cyanobacterial cells activated photoprotective mechanisms such as photoinhibition and non-photochemical quenching. Later on (during the second phase), we temporarily observed functional decoupling of phycobilisomes and sustained monomerization of photosystem II dimer. Simultaneously, cells also initiated accumulation of carotenoids, especially gamma-carotene, the main precursor of all carotenoids. In the last phase, in addition to gamma-carotene, we also observed accumulation of myxoxanthophyll and more even spatial distribution of photosystems and phycobilisomes between microdomains. We suggest that the overall carotenoid increase during HL stress could be involved either in the direct photoprotection (e.g., in ROS scavenging) and/or could play an additional role in maintaining optimal distribution of photosystems in thylakoid membrane to attain efficient photoprotection.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2022
    Elektronická adresahttps://www.mdpi.com/2073-4409/10/8/1916
Počet záznamů: 1  

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