Počet záznamů: 1
In Vitro Interactions of TiO2 Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment
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SYSNO ASEP 0541602 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název In Vitro Interactions of TiO2 Nanoparticles with Earthworm Coelomocytes: Immunotoxicity Assessment Tvůrce(i) Navarro Pacheco, Natividad Isabel (MBU-M) ORCID
Roubalová, Radka (MBU-M) RID, ORCID
Semerád, Jaroslav (MBU-M) ORCID, RID
Grasserová, Alena (MBU-M) ORCID
Benada, Oldřich (MBU-M) ORCID, RID
Kofroňová, Olga (MBU-M) RID, ORCID
Cajthaml, Tomáš (MBU-M) RID, ORCID
Dvořák, Jiří (MBU-M) RID, ORCID
Bilej, Martin (MBU-M) RID, ORCID
Procházková, Petra (MBU-M) RID, ORCIDČíslo článku 250 Zdroj.dok. Nanomaterials. - : MDPI
Roč. 11, č. 1 (2021)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova earthworm ; coelomocyte ; TiO2 nanoparticles ; reactive oxygen species ; innate immunity ; lipid peroxidation Vědní obor RIV DJ - Znečištění a kontrola vody Obor OECD Environmental sciences (social aspects to be 5.7) CEP QK1910095 GA MZe - Ministerstvo zemědělství Výzkumná infrastruktura Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000611050100001 EID SCOPUS 85100141143 DOI 10.3390/nano11010250 Anotace Titanium dioxide nanoparticles (TiO2 NPs) are manufactured worldwide. Once they arrive in the soil environment, they can endanger living organisms. Hence, monitoring and assessing the effects of these nanoparticles is required. We focus on the Eisenia andrei earthworm immune cells exposed to sublethal concentrations of TiO2 NPs (1, 10, and 100 mu g/mL) for 2, 6, and 24 h. TiO2 NPs at all concentrations did not affect cell viability. Further, TiO2 NPs did not cause changes in reactive oxygen species (ROS) production, malondialdehyde (MDA) production, and phagocytic activity. Similarly, they did not elicit DNA damage. Overall, we did not detect any toxic effects of TiO2 NPs at the cellular level. At the gene expression level, slight changes were detected. Metallothionein, fetidin/lysenin, lumbricin and MEK kinase I were upregulated in coelomocytes after exposure to 10 mu g/mL TiO2 NPs for 6 h. Antioxidant enzyme expression was similar in exposed and control cells. TiO2 NPs were detected on coelomocyte membranes. However, our results do not show any strong effects of these nanoparticles on coelomocytes at both the cellular and molecular levels. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2079-4991/11/1/250
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