Počet záznamů: 1
Protein downstream processing: Design, Development And Application of High and Low-Resolution Methods (N. Labrou Ed.) 2nd edition
- 1.
SYSNO ASEP 0540021 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Stabilization of proteins by freeze-drying in the presence of trehalose: a case study of tubulin Tvůrce(i) Dráber, Pavel (UMG-J) RID, ORCID
Sulimenko, Tetyana (UMG-J)
Sulimenko, Vadym (UMG-J) RID, ORCID
Dráberová, Eduarda (UMG-J) RID, ORCIDZdroj.dok. Protein downstream processing: Design, Development And Application of High and Low-Resolution Methods (N. Labrou Ed.) 2nd edition, Stabilization of proteins by freeze-drying in the presence of trehalose: a case study of tubulin. - New York : Humana Press, 2021 - ISBN 978-1-0716-0775-6 Rozsah stran s. 417-435 Poč.str. 19 s. Poč.str.knihy 511 Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova freeze-drying ; trehalose ; tubulin Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP GA18-27197S GA ČR - Grantová agentura ČR GA19-20716S GA ČR - Grantová agentura ČR LTAUSA17052 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMG-J - RVO:68378050 UT WOS 000697782600028 DOI 10.1007/978-1-0716-0775-6_27 Anotace Microtubules, polymers of the heterodimeric protein αβ-tubulin, are indispensable for many cellular
activities such as maintenance of cell shape, division, migration, and ordered vesicle transport. In vitro
assays to study microtubule functions and their regulation by associated proteins require the availability of assembly-competent purified tubulin. However, tubulin is a thermolabile protein that rapidly converts into a nonpolymerizing state. For this reason, it is usually stored at -80 C or liquid nitrogen to preserve its conformation and polymerization properties. In this chapter, we describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose, and methods enabling the evaluation of tubulin functions in rehydrated samples.
Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2023 Elektronická adresa https://link.springer.com/protocol/10.1007%2F978-1-0716-0775-6_27
Počet záznamů: 1