cDNA Library Screening Identifies Protein Interactors Potentially Involved in Non-Telomeric Roles of Arabidopsis Telomerase

0456689 - BFÚ 2016 RIV CH eng J - Článek v odborném periodiku
Dokládal, Ladislav - Honys, David - Rana, Rajiv - Lee, L.-Y. - Gelvin, S.B. - Sýkorová, Eva
cDNA Library Screening Identifies Protein Interactors Potentially Involved in Non-Telomeric Roles of Arabidopsis Telomerase.
Frontiers in Plant Science. Roč. 6, NOV2015 (2015). ISSN 1664-462X. E-ISSN 1664-462X
Grant CEP: GA ČR GA13-06943S; GA MŠMT(CZ) ED1.1.00/02.0068
Grant ostatní: GA MŠk(CZ) LH10352
Institucionální podpora: RVO:68081707 ; RVO:61389030
Klíčová slova: telomerase * nuclear poly(A)-binding protein * telobox
Kód oboru RIV: BO - Biofyzika; EF - Botanika (UEB-Q)
Impakt faktor: 4.495, rok: 2015

Telomerase-reverse transcriptase (TERT) plays an essential catalytic role in maintaining telomeres. However, in animal systems telomerase plays additional non-telomeric functional roles. We previously screened an Arabidopsis cDNA library for proteins that interact with the C-terminal extension (GTE) TERT domain and identified a nuclearlocalized protein that contains an RNA recognition motif (RRM). This RRM-protein forms homodimers in both plants and yeast. Mutation of the gene encoding the RRM-protein had no detectable effect on plant growth and development, nor did it affect telomerase activity or telomere length in vivo, suggesting a non-telomeric role for TERT/RRM-protein complexes. The gene encoding the RRM-protein is highly expressed in leaf and reproductive tissues. We further screened an Arabiclopsis cDNA library for proteins that interact with the RRM-protein and identified five interactors. These proteins are involved in numerous non-telomere-associated cellular activities.
Trvalý link: http://hdl.handle.net/11104/0257193