Lipid Profiling in Epicardial and Subcutaneous Adipose Tissue of Patients with Coronary Artery Disease

0536067 - MBÚ 2021 RIV US eng J - Článek v odborném periodiku
Tomášová, Petra - Čermáková, Martina - Pelantová, Helena - Vecka, M. - Kratochvílová, H. - Lipš, M. - Lindner, J. - Ivák, P. - Netuka, I. - Šedivá, Blanka - Haluzník, M. - Kuzma, Marek
Lipid Profiling in Epicardial and Subcutaneous Adipose Tissue of Patients with Coronary Artery Disease.
Journal of Proteome Research. Roč. 19, č. 10 (2020), s. 3993-4003. ISSN 1535-3893. E-ISSN 1535-3907
Grant CEP: GA MŠMT(CZ) LO1509
Institucionální podpora: RVO:61388971
Klíčová slova: epicardial adipose tissue * subcutaneous adipose tissue * coronary artery disease
Obor OECD: Microbiology
Impakt faktor: 4.466, rok: 2020
Způsob publikování: Omezený přístup
https://pubs.acs.org/doi/abs/10.1021/acs.jproteome.0c00269

Coronary artery disease is one of the most frequent causes of morbidity and mortality worldwide. It is even more prevalent in patients with type 2 diabetes mellitus who suffer from obesity and increased accumulation of epicardial fat with a possible contributing role in the development of coronary artery disease. We performed an MS-based lipidomic analysis of subcutaneous and epicardial adipose tissue in 23 patients with coronary artery disease stratified for the presence/absence of type 2 diabetes mellitus and a control group of 13 subjects aiming at identification of factors from epicardial fat contributing to the development of coronary artery disease. The samples of adipose tissues were obtained during elective cardiac surgery. They were extracted and analyzed with and without previous triacylglycerols separation by high-pressure liquid chromatography-mass spectrometry (HPLC-MS). Multi-variate and univariate analyses were performed. Lipidomics data were correlated with biochemical parameters. We identified multiple changes in monoacylglycerols, diacylglycerols, triacylglycerols, glycerophosphatidylserines, glycerophosphatidylethanolamines, glycerophosphatidylcholines, ceramides, sphingomyelins, and derivatives of cholesterol. Observed changes included molecules with fatty acids with odd (15:0, 15:1, 17:0, 17:1) and even (10:0, 12:0, 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 20:4, 20:1, 22:0) fatty acids in both types of adipose tissue. More pronounced changes were detected in epicardial adipose tissue compared to subcutaneous adipose tissue of patients with coronary artery disease and type 2 diabetes. Lipidomic analysis of subcutaneous and epicardial adipose tissue revealed different profiles for patients with coronary artery disease and type 2 diabetes, which might be related to coronary artery disease and the presence of type 2 diabetes.
Trvalý link: http://hdl.handle.net/11104/0313916