Determination of effective charges and ionic mobilities of polycationic antimicrobial peptides by capillary isotachophoresis and capillary zone electrophoresis

0478419 - ÚOCHB 2018 RIV DE eng J - Článek v odborném periodiku
Tůmová, Tereza - Monincová, Lenka - Nešuta, Ondřej - Čeřovský, Václav - Kašička, Václav
Determination of effective charges and ionic mobilities of polycationic antimicrobial peptides by capillary isotachophoresis and capillary zone electrophoresis.
Electrophoresis. Roč. 38, č. 16 (2017), s. 2018-2024. ISSN 0173-0835. E-ISSN 1522-2683
Grant CEP: GA ČR(CZ) GA15-01948S
Institucionální podpora: RVO:61388963
Klíčová slova: antimicrobial peptides * capillary isotachophoresis * counterion condensation * effective charge * ionic mobility
Obor OECD: Analytical chemistry
Impakt faktor: 2.569, rok: 2017

Capillary ITP (CITP) and CZE were applied to the determination of effective charges and ionic mobilities of polycationic antimicrobial peptides (AMPs). Twelve AMPs (deca- to hexadecapeptides) containing three to seven basic amino acid residues (His, Lys, Arg) at variable positions of peptide chain were investigated. Effective charges of the AMPs were determined from the lengths of their ITP zones, ionic mobilities, and molar concentrations, and from the same parameters of the reference compounds. Lengths of the ITP zones of AMPs and reference compounds were obtained from their CITP analyses in cationic mode using leading electrolyte (LE) composed of 10mMNH(4)OH, 40mMAcOH (acetic acid), pH 4.1, and terminating electrolyte (TE) containing 40mMAcOH, pH 3.2. Ionic mobilities of AMPs and singly charged reference compounds (ammediol or arginine) were determined by their CZE analyses in the BGE of the same composition as the LE. The effective charges numbers of AMPs were found to be in the range 1.65-5.04, i.e. significantly reduced as compared to the theoretical charge numbers (2.86-6.99) calculated from the acidity constants of the analyzed AMPs. This reduction of effective charge due to tightly bound acetate counterions (counterion condensation) was in the range 17-47% depending on the number and type of the basic amino acid residues in the AMPs molecules. Ionic mobilities of AMPs achieved values (26.5-38.6)x10(-9)m(2)V(-1)s(-1) and in most cases were in a good agreement with the ratio of their effective charges and relative molecular masses.
Trvalý link: http://hdl.handle.net/11104/0274538